Literature DB >> 2843361

Cloning and characterization of a DNA gyrase B gene from Streptomyces sphaeroides that confers resistance to novobiocin.

A S Thiara1, E Cundliffe.   

Abstract

A gyrB gene from Streptomyces sphaeroides, a producer of novobiocin, has been cloned in Streptomyces lividans, where it conferred resistance to novobiocin. The Streptomyces gyrB gene was sufficiently similar to a Bacillus subtilis gyrB probe to be specifically recognized during Southern analysis. Partial purification of DNA gyrase by affinity chromatography revealed the presence of two such activities (differing in their responses to novobiocin) in the clone. The product of the cloned gene, a novobiocin-resistant DNA gyrase B subunit, was identified in vitro by coupled transcription--translation as a 79-kd protein.

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Year:  1988        PMID： 2843361      PMCID： PMC454581          DOI： 10.1002/j.1460-2075.1988.tb03065.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  28 in total

1.  Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

Authors:  A Sugino; C L Peebles; K N Kreuzer; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

2.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

3.  Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.

Authors:  S L Swanberg; J C Wang
Journal:  J Mol Biol       Date:  1987-10-20       Impact factor: 5.469

4.  Energy coupling in DNA gyrase and the mechanism of action of novobiocin.

Authors:  A Sugino; N P Higgins; P O Brown; C L Peebles; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

5.  Effect of rifampicin on in vitro RNA synthesis of Streptomyces mediterranei.

Authors:  S Watanabe; K Tanaka
Journal:  Biochem Biophys Res Commun       Date:  1976-09-20       Impact factor: 3.575

6.  Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis.

Authors:  M F Lampe; K F Bott
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

7.  Cerulenin resistance in a cerulenin-producing fungus. Isolation of cerulenin insensitive fatty acid synthetase.

Authors:  A Kawaguchi; H Tomoda; S Okuda; J Awaya; S Omura
Journal:  Arch Biochem Biophys       Date:  1979-10-01       Impact factor: 4.013

8.  Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity.

Authors:  N P Higgins; C L Peebles; A Sugino; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

9.  DNA gyrase: subunit structure and ATPase activity of the purified enzyme.

Authors:  K Mizuuchi; M H O'Dea; M Gellert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

10.  Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; T Itoh; J I Tomizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205
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  20 in total

Review 1.  Avoidance of suicide in antibiotic-producing microbes.

Authors:  Eric Cundliffe; Arnold L Demain
Journal:  J Ind Microbiol Biotechnol       Date:  2010-05-06       Impact factor: 3.346

2.  Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria.

Authors:  M L Holmes; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

3.  GyrB mutations in Staphylococcus aureus strains resistant to cyclothialidine, coumermycin, and novobiocin.

Authors:  M Stieger; P Angehrn; B Wohlgensinger; H Gmünder
Journal:  Antimicrob Agents Chemother       Date:  1996-04       Impact factor: 5.191

Review 4.  Biochemical characteristics and physiological significance of major DNA topoisomerases.

Authors:  J A Sutcliffe; T D Gootz; J F Barrett
Journal:  Antimicrob Agents Chemother       Date:  1989-12       Impact factor: 5.191

5.  Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.

Authors:  M Steffensky; A Mühlenweg; Z X Wang; S M Li; L Heide
Journal:  Antimicrob Agents Chemother       Date:  2000-05       Impact factor: 5.191

6.  Sequence analysis, purification, and study of inhibition by 4-quinolones of the DNA gyrase from Mycobacterium smegmatis.

Authors:  V Revel-Viravau; Q C Truong; N Moreau; V Jarlier; W Sougakoff
Journal:  Antimicrob Agents Chemother       Date:  1996-09       Impact factor: 5.191

7.  Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations.

Authors:  H E Takiff; L Salazar; C Guerrero; W Philipp; W M Huang; B Kreiswirth; S T Cole; W R Jacobs; A Telenti
Journal:  Antimicrob Agents Chemother       Date:  1994-04       Impact factor: 5.191

8.  Antibacterial activity of and resistance to small molecule inhibitors of the ClpP peptidase.

Authors:  Corey L Compton; Karl R Schmitz; Robert T Sauer; Jason K Sello
Journal:  ACS Chem Biol       Date:  2013-10-04       Impact factor: 5.100

9.  Targeted inhibition of heat shock protein 90 suppresses tumor necrosis factor-α and ameliorates murine intestinal inflammation.

Authors:  Colm B Collins; Derek Strassheim; Carol M Aherne; Alyson R Yeckes; Paul Jedlicka; Edwin F de Zoeten
Journal:  Inflamm Bowel Dis       Date:  2014-04       Impact factor: 5.325

10.  Andrimid producers encode an acetyl-CoA carboxyltransferase subunit resistant to the action of the antibiotic.

Authors:  Xinyu Liu; Pascal D Fortin; Christopher T Walsh
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-03       Impact factor: 11.205
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