Literature DB >> 2828631

Cloning and sequencing of the Escherichia coli gyrA gene coding for the A subunit of DNA gyrase.

S L Swanberg1, J C Wang.   

Abstract

The gene gyrA of Escherichia coli, which encodes the A subunit of DNA gyrase (topoisomerase II), has been cloned and a region of approximately 3300 base-pairs sequenced. An open reading frame of 2625 nucleotides coding for a protein of 97,000 Mr is located. The peptide weight of the subunit predicted from this open reading frame is in close agreement with previously published estimates of that of the A subunit. There is a "TATAAT" promoter motif located 44 bases upstream from the first "ATG" of the open reading frame. The amino acid sequence derived from the nucleotide sequence is about 50% homologous with that derived from the Bacillus subtilis gyrA gene sequence, with several regions showing greater than 90% homology.

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Year:  1987        PMID： 2828631     DOI： 10.1016/0022-2836(87)90479-7

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  69 in total

1.  Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes.

Authors:  J McKinney; C Guerrier-Takada; D Wesolowski; S Altman
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-29       Impact factor: 11.205

2.  Specific gyrA mutation at codon 83 in nalidixic acid-resistant Salmonella enterica serovar Typhi strains isolated from Vietnamese patients.

Authors:  Le Van Phung; Haruko Ryo; Taisei Nomura
Journal:  Antimicrob Agents Chemother       Date:  2002-06       Impact factor: 5.191

Review 3.  Quinolone antimicrobial agents: adverse effects and bacterial resistance.

Authors:  J S Wolfson
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-12       Impact factor: 3.267

4.  Development of a rapid assay for detecting gyrA mutations in Escherichia coli and determination of incidence of gyrA mutations in clinical strains isolated from patients with complicated urinary tract infections.

Authors:  S Ozeki; T Deguchi; M Yasuda; M Nakano; T Kawamura; Y Nishino; Y Kawada
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

5.  Sequence analysis of the gyrA and parC homologues of a wild-type strain of Vibrio parahaemolyticus and its fluoroquinolone-resistant mutants.

Authors:  J Okuda; E Hayakawa; M Nishibuchi; T Nishino
Journal:  Antimicrob Agents Chemother       Date:  1999-05       Impact factor: 5.191

6.  Pre-boiling high GC content, mixed primers with 3' complementation allows the successful PCR amplification of Pseudomonas aeruginosa DNA.

Authors:  A Kureishi; L E Bryan
Journal:  Nucleic Acids Res       Date:  1992-03-11       Impact factor: 16.971

7.  Location of gyrA on the physical map of the Escherichia coli chromosome.

Authors:  G Webb; K Rohatgi; J B Courtright
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

8.  Mapping the active-site tyrosine of vaccinia virus DNA topoisomerase I.

Authors:  S Shuman; E M Kane; S G Morham
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

9.  DNA gyrase and topoisomerase IV mutations associated with fluoroquinolone resistance in Proteus mirabilis.

Authors:  L M Weigel; G J Anderson; F C Tenover
Journal:  Antimicrob Agents Chemother       Date:  2002-08       Impact factor: 5.191

10.  Limitations of plasmid complementation test for determination of quinolone resistance due to changes in the gyrase A protein and identification of conditional quinolone resistance locus.

Authors:  C J Soussy; J S Wolfson; E Y Ng; D C Hooper
Journal:  Antimicrob Agents Chemother       Date:  1993-12       Impact factor: 5.191
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