Literature DB >> 2840639

Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.

L E Pyle1, L N Corcoran, B G Cocks, A D Bergemann, J C Whitley, L R Finch.   

Abstract

The sizes of large DNA fragments produced from genomes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides, GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.

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Year:  1988        PMID： 2840639      PMCID： PMC336844          DOI： 10.1093/nar/16.13.6015

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Restriction endonucleases for pulsed field mapping of bacterial genomes.

Authors:  M McClelland; R Jones; Y Patel; M Nelson
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

2.  Chromosome analysis by two-dimensional fingerprinting.

Authors:  S K Poddar; J Maniloff
Journal:  Gene       Date:  1986       Impact factor: 3.688

Review 3.  Genetic map of Saccharomyces cerevisiae, edition 9.

Authors:  R K Mortimer; D Schild
Journal:  Microbiol Rev       Date:  1985-09

4.  Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment.

Authors:  E G Niles; R C Condit; P Caro; K Davidson; L Matusick; J Seto
Journal:  Virology       Date:  1986-08       Impact factor: 3.616

5.  Separation of large DNA molecules by contour-clamped homogeneous electric fields.

Authors:  G Chu; D Vollrath; R W Davis
Journal:  Science       Date:  1986-12-19       Impact factor: 47.728

6.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

7.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

Review 8.  Molecular biology and genetics of mycoplasmas (Mollicutes).

Authors:  S Razin
Journal:  Microbiol Rev       Date:  1985-12

9.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum.

Authors:  D J Kemp; L M Corcoran; R L Coppel; H D Stahl; A E Bianco; G V Brown; R F Anders
Journal:  Nature       Date:  1985 May 23-29       Impact factor: 49.962
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  37 in total

1.  Construction of an EcoRI restriction map of Mycoplasma pneumoniae and localization of selected genes.

Authors:  R Wenzel; E Pirkl; R Herrmann
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

2.  Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.

Authors:  D C Krause; C B Mawn
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

3.  Analysis of three different repeated DNA elements present in the P1 operon of Mycoplasma pneumoniae: size, number and distribution on the genome.

Authors:  K Ruland; R Wenzel; R Herrmann
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

4.  A systematic study of field inversion gel electrophoresis.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1989-08-11       Impact factor: 16.971

Review 5.  New approaches for physical mapping of small genomes.

Authors:  C L Smith; G Condemine
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

6.  Determination of genome size of Pseudomonas aeruginosa by PFGE: analysis of restriction fragments.

Authors:  J S Hector; A R Johnson
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

7.  Physical and genetic mapping of the Rhodobacter sphaeroides 2.4.1 genome: genome size, fragment identification, and gene localization.

Authors:  A Suwanto; S Kaplan
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

8.  A physical map of the genome of Ureaplasma urealyticum 960T with ribosomal RNA loci.

Authors:  B G Cocks; L E Pyle; L R Finch
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971

9.  Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.

Authors:  W Bautsch
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

10.  In vivo damage and recA-dependent repair of plasmid and chromosomal DNA in the radiation-resistant bacterium Deinococcus radiodurans.

Authors:  M J Daly; L Ouyang; P Fuchs; K W Minton
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490
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