Literature DB >> 3264905

Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.

W Bautsch1.   

Abstract

A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzymes could be assigned to the map. These rapid methods do not require DNA probes and are applicable to the long-range restriction mapping of all genomes that yield resolvable patterns on two-dimensional gels.

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Year:  1988        PMID： 3264905      PMCID： PMC339058          DOI： 10.1093/nar/16.24.11461

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Restriction endonucleases for pulsed field mapping of bacterial genomes.

Authors:  M McClelland; R Jones; Y Patel; M Nelson
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

2.  Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.

Authors:  L E Pyle; L N Corcoran; B G Cocks; A D Bergemann; J C Whitley; L R Finch
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

3.  A physical map of the Escherichia coli K12 genome.

Authors:  C L Smith; J G Econome; A Schutt; S Klco; C R Cantor
Journal:  Science       Date:  1987-06-12       Impact factor: 47.728

4.  Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor gamma gene family.

Authors:  T Woolf; E Lai; M Kronenberg; L Hood
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

5.  Genome size of mycoplasmal DNA.

Authors:  A L Bak; F T Black; C Christiansen; E A Freundt
Journal:  Nature       Date:  1969-12-20       Impact factor: 49.962

6.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

7.  Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.

Authors:  G Darai; L Zöller; B Matz; H Delius; P T Speck; R M Flügel
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

8.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

9.  A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.

Authors:  L E Pyle; L R Finch
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

10.  Isolation of a motile mycoplasma from fish.

Authors:  H Kirchhoff; R Rosengarten
Journal:  J Gen Microbiol       Date:  1984-09
  10 in total
  35 in total

1.  In-gel DNA radiolabelling and two-dimensional pulsed field gel electrophoresis procedures suitable for fingerprinting and mapping small eukaryotic genomes.

Authors:  J F Brugère; E Cornillot; G Méténier; C P Vivarès
Journal:  Nucleic Acids Res       Date:  2000-05-15       Impact factor: 16.971

2.  Physical map of the linear chromosome of Streptomyces hygroscopicus 10-22 deduced by analysis of overlapping large chromosomal deletions.

Authors:  Xiuhua Pang; Xiufen Zhou; Yuhui Sun; Zixin Deng
Journal:  J Bacteriol       Date:  2002-04       Impact factor: 3.490

3.  Construction of an EcoRI restriction map of Mycoplasma pneumoniae and localization of selected genes.

Authors:  R Wenzel; E Pirkl; R Herrmann
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

4.  A physical map of the Salmonella typhimurium LT2 genome made by using XbaI analysis.

Authors:  S L Liu; K E Sanderson
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

5.  Physical analysis and mapping of the Mycoplasma pneumoniae chromosome.

Authors:  D C Krause; C B Mawn
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

6.  A physical map for Mycoplasma capricolum Cal. kid with loci for all known tRNA species.

Authors:  J C Whitley; A Muto; L R Finch
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

7.  The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.

Authors:  U Römling; B Tümmler
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

Review 8.  New approaches for physical mapping of small genomes.

Authors:  C L Smith; G Condemine
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

9.  Genome analysis of Bradyrhizobium japonicum serocluster 123 field isolates by using field inversion gel electrophoresis.

Authors:  B W Sobral; M J Sadowsky; A G Atherly
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

10.  Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

Authors:  E Tigges; F C Minion
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490
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