Maria Gabriella Gabrielli1, Daniele Tomassoni2. 1. School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, I, Camerino, 62032, Macerata, Italy. gabriella.gabrielli@unicam.it. 2. School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, I, Camerino, 62032, Macerata, Italy.
Abstract
PURPOSE: The protective function of the intestinal mucosa largely depends on carbohydrate moieties that as a part of glycoproteins and glycolipids form the epithelial glycocalyx or are secreted as mucins. Modifications of their expression can be induced by an altered intestinal microenvironment and have been associated with inflammatory disorders and colorectal cancer. Given the influence of dietary factors on the gut ecosystem, here we have investigated whether a long term feeding on a starch-rich diet can modulate the glucidic profile in the colonic mucosa of rats. METHODS: Animals were divided into two groups and maintained for 9 months at different diets: one group was fed a standard diet, the second was fed a starch-enriched diet. Samples of colonic mucosa, divided in proximal and distal portions, were processed for microscopic analysis. Conventional stainings and lectin histochemistry were applied to identify acidic glycoconjugates and specific sugar residues in oligosaccharide chains, respectively. Some lectins were applied on adjacent sections after sialidase/fucosidase digestion, deacetylation, and oxidation to characterize either terminal dimers or sialic acid acetylation. RESULTS: An increase in sulfomucins was found to be associated with the starch-enriched diet that affected also the expression of several sugar residues as well as fucosylated and sialylated sequences in both proximal and distal colon. CONCLUSIONS: Although the mechanisms leading to such a modulation are at present unknown, either an altered intestinal microbiota or a dysregulation of glycosylation patterns might be responsible for the types and distribution of changes in the glucidic profile here observed.
PURPOSE: The protective function of the intestinal mucosa largely depends on carbohydrate moieties that as a part of glycoproteins and glycolipids form the epithelial glycocalyx or are secreted as mucins. Modifications of their expression can be induced by an altered intestinal microenvironment and have been associated with inflammatory disorders and colorectal cancer. Given the influence of dietary factors on the gut ecosystem, here we have investigated whether a long term feeding on a starch-rich diet can modulate the glucidic profile in the colonic mucosa of rats. METHODS: Animals were divided into two groups and maintained for 9 months at different diets: one group was fed a standard diet, the second was fed a starch-enriched diet. Samples of colonic mucosa, divided in proximal and distal portions, were processed for microscopic analysis. Conventional stainings and lectin histochemistry were applied to identify acidic glycoconjugates and specific sugar residues in oligosaccharide chains, respectively. Some lectins were applied on adjacent sections after sialidase/fucosidase digestion, deacetylation, and oxidation to characterize either terminal dimers or sialic acid acetylation. RESULTS: An increase in sulfomucins was found to be associated with the starch-enriched diet that affected also the expression of several sugar residues as well as fucosylated and sialylated sequences in both proximal and distal colon. CONCLUSIONS: Although the mechanisms leading to such a modulation are at present unknown, either an altered intestinal microbiota or a dysregulation of glycosylation patterns might be responsible for the types and distribution of changes in the glucidic profile here observed.
Authors: Francesca Turroni; Francesca Bottacini; Elena Foroni; Imke Mulder; Jae-Han Kim; Aldert Zomer; Borja Sánchez; Alessandro Bidossi; Alberto Ferrarini; Vanessa Giubellini; Massimo Delledonne; Bernard Henrissat; Pedro Coutinho; Marco Oggioni; Gerald F Fitzgerald; David Mills; Abelardo Margolles; Denise Kelly; Douwe van Sinderen; Marco Ventura Journal: Proc Natl Acad Sci U S A Date: 2010-10-25 Impact factor: 11.205
Authors: Eirikur Saeland; Ana I Belo; Sandra Mongera; Irma van Die; Gerrit A Meijer; Yvette van Kooyk Journal: Int J Cancer Date: 2011-11-28 Impact factor: 7.396
Authors: Joseph M Pickard; Corinne F Maurice; Melissa A Kinnebrew; Michael C Abt; Dominik Schenten; Tatyana V Golovkina; Said R Bogatyrev; Rustem F Ismagilov; Eric G Pamer; Peter J Turnbaugh; Alexander V Chervonsky Journal: Nature Date: 2014-10-01 Impact factor: 49.962
Authors: Guangyu An; Bo Wei; Baoyun Xia; J Michael McDaniel; Tongzhong Ju; Richard D Cummings; Jonathan Braun; Lijun Xia Journal: J Exp Med Date: 2007-05-21 Impact factor: 14.307