BACKGROUND: Functional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects. METHODS: Unclassified patients (n=46) and controls (n=25) were evaluated for T-cell disorders with CFSE-based assay. RESULTS: CD3+ blast cells after PHA-L stimulation were significantly lower in patients (31.1±28.8) than controls (67.9±8.79; P<.001). Nine patients with low and four patients with normal CD3 values had severely decreased blastic transformation. The proliferation response decreased mostly in combined immunodeficiency group. Sixteen of them had impaired proliferation responses. Appropriate molecular genetical analyses were planned after thorough evaluation of each patient. CONCLUSIONS: In vitro lymphocyte cell proliferation analysis by CFSE method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified PID patients for targeting further genetical analyses.
BACKGROUND: Functional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects. METHODS: Unclassified patients (n=46) and controls (n=25) were evaluated for T-cell disorders with CFSE-based assay. RESULTS: CD3+ blast cells after PHA-L stimulation were significantly lower in patients (31.1±28.8) than controls (67.9±8.79; P<.001). Nine patients with low and four patients with normal CD3 values had severely decreased blastic transformation. The proliferation response decreased mostly in combined immunodeficiency group. Sixteen of them had impaired proliferation responses. Appropriate molecular genetical analyses were planned after thorough evaluation of each patient. CONCLUSIONS: In vitro lymphocyte cell proliferation analysis by CFSE method is a reliable and practical choice for the assessment of mitogenic T lymphocyte responses in yet unclassified PID patients for targeting further genetical analyses.
Authors: Jamie A Lee; Josef Spidlen; Keith Boyce; Jennifer Cai; Nicholas Crosbie; Mark Dalphin; Jeff Furlong; Maura Gasparetto; Michael Goldberg; Elizabeth M Goralczyk; Bill Hyun; Kirstin Jansen; Tobias Kollmann; Megan Kong; Robert Leif; Shannon McWeeney; Thomas D Moloshok; Wayne Moore; Garry Nolan; John Nolan; Janko Nikolich-Zugich; David Parrish; Barclay Purcell; Yu Qian; Biruntha Selvaraj; Clayton Smith; Olga Tchuvatkina; Anne Wertheimer; Peter Wilkinson; Christopher Wilson; James Wood; Robert Zigon; Richard H Scheuermann; Ryan R Brinkman Journal: Cytometry A Date: 2008-10 Impact factor: 4.355
Authors: Capucine Picard; Waleed Al-Herz; Aziz Bousfiha; Jean-Laurent Casanova; Talal Chatila; Mary Ellen Conley; Charlotte Cunningham-Rundles; Amos Etzioni; Steven M Holland; Christoph Klein; Shigeaki Nonoyama; Hans D Ochs; Eric Oksenhendler; Jennifer M Puck; Kathleen E Sullivan; Mimi L K Tang; Jose Luis Franco; H Bobby Gaspar Journal: J Clin Immunol Date: 2015-10-19 Impact factor: 8.317