Govindarajan Srimathveeravalli1, Francois Cornelis2, Thomas Wimmer3, Sebastien Monette4, Simon Y Kimm5, Majid Maybody6, Stephen B Solomon6, Jonathan A Coleman7, Jeremy C Durack6. 1. Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York; Weill Cornell Medical College, New York, New York. Electronic address: srimaths@mskcc.org. 2. Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York; Tenon Hospital, Assistance Publique-Hôpitaux de Paris Pierre & Marie Curie University, Paris, France. 3. Medical University of Graz, Graz, Austria. 4. Laboratory of Comparative Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York; The Rockefeller University, Weill Cornell Medicine, New York, New York. 5. Department of Urology, Palo Alto Medical Foundation, Palo Alto, California. 6. Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, New York. 7. Department of Surgery, Division of Urology, Memorial Sloan-Kettering Cancer Center, New York, New York.
Abstract
PURPOSE: To evaluate the effect of catheter-directed irreversible electroporation (IRE) on the integrity, patency, and function of the normal porcine ureter. MATERIALS AND METHODS: A catheter-mounted electrode was used to perform fluoroscopy-guided IRE in 8 healthy pigs. Two unilateral ablations (90 pulses at 2,000 V, 100 μs) were performed in each animal in the proximal and distal ureter. Serum creatinine measurements and contrast-enhanced computed tomography imaging were performed at 1, 7, 14, 21, and 28 days after IRE, and findings were compared with baseline values by Student t test. Two animals each were euthanized at 1, 7, 14, and 28 days after IRE for histologic assessment of treatment effects. Quantitative histologic analysis of regeneration and healing of the ureteral wall was graded on a five-point scale. RESULTS: IRE was successfully performed in all animals. Preservation of ureteral wall integrity was confirmed by the leakage-free passage of contrast medium in the treated ureter of all animals through the observation period. Ureteral strictures and associated renal pelvicaliceal dilation were observed in all animals by study days 7 (P = .005) and 14 (P = .007) and did not resolve by day 28. Urothelial recovery was observed in tissue samples from day 7, with progressive replacement of the tunica muscularis with granulation tissue. Despite extensive scarring of the tunica muscularis, full recovery of the urothelium was observed by day 28. CONCLUSIONS: The normal porcine ureter retains lumen wall integrity and function following catheter-directed IRE. Scarring of the tunica muscularis in the treated ureter results in stricture formation and reduction of lumen patency.
PURPOSE: To evaluate the effect of catheter-directed irreversible electroporation (IRE) on the integrity, patency, and function of the normal porcine ureter. MATERIALS AND METHODS: A catheter-mounted electrode was used to perform fluoroscopy-guided IRE in 8 healthy pigs. Two unilateral ablations (90 pulses at 2,000 V, 100 μs) were performed in each animal in the proximal and distal ureter. Serum creatinine measurements and contrast-enhanced computed tomography imaging were performed at 1, 7, 14, 21, and 28 days after IRE, and findings were compared with baseline values by Student t test. Two animals each were euthanized at 1, 7, 14, and 28 days after IRE for histologic assessment of treatment effects. Quantitative histologic analysis of regeneration and healing of the ureteral wall was graded on a five-point scale. RESULTS: IRE was successfully performed in all animals. Preservation of ureteral wall integrity was confirmed by the leakage-free passage of contrast medium in the treated ureter of all animals through the observation period. Ureteral strictures and associated renal pelvicaliceal dilation were observed in all animals by study days 7 (P = .005) and 14 (P = .007) and did not resolve by day 28. Urothelial recovery was observed in tissue samples from day 7, with progressive replacement of the tunica muscularis with granulation tissue. Despite extensive scarring of the tunica muscularis, full recovery of the urothelium was observed by day 28. CONCLUSIONS: The normal porcine ureter retains lumen wall integrity and function following catheter-directed IRE. Scarring of the tunica muscularis in the treated ureter results in stricture formation and reduction of lumen patency.
Authors: Morgan Rouprêt; Marko Babjuk; Eva Compérat; Richard Zigeuner; Richard Sylvester; Max Burger; Nigel Cowan; Andreas Böhle; Bas W G Van Rhijn; Eero Kaasinen; Joan Palou; Shahrokh F Shariat Journal: Eur Urol Date: 2013-03-19 Impact factor: 20.096
Authors: Muneeb Ahmed; Luigi Solbiati; Christopher L Brace; David J Breen; Matthew R Callstrom; J William Charboneau; Min-Hua Chen; Byung Ihn Choi; Thierry de Baère; Gerald D Dodd; Damian E Dupuy; Debra A Gervais; David Gianfelice; Alice R Gillams; Fred T Lee; Edward Leen; Riccardo Lencioni; Peter J Littrup; Tito Livraghi; David S Lu; John P McGahan; Maria Franca Meloni; Boris Nikolic; Philippe L Pereira; Ping Liang; Hyunchul Rhim; Steven C Rose; Riad Salem; Constantinos T Sofocleous; Stephen B Solomon; Michael C Soulen; Masatoshi Tanaka; Thomas J Vogl; Bradford J Wood; S Nahum Goldberg Journal: J Vasc Interv Radiol Date: 2014-10-23 Impact factor: 3.464
Authors: Govindarajan Srimathveeravalli; Mikhail Silk; Thomas Wimmer; Sebastien Monette; Simon Kimm; Majid Maybody; Stephen B Solomon; Jonathan Coleman; Jeremy C Durack Journal: J Vasc Interv Radiol Date: 2015-03-11 Impact factor: 3.464
Authors: Markus J Bader; Ronald Sroka; Christian Gratzke; Michael Seitz; Patrick Weidlich; Michael Staehler; Armin Becker; Christian G Stief; Oliver Reich Journal: Eur Urol Date: 2008-12-17 Impact factor: 20.096
Authors: R Yakoubi; P Colin; T Seisen; P Léon; L Nison; G Bozzini; S F Shariat; M Rouprêt Journal: Eur J Surg Oncol Date: 2014-07-25 Impact factor: 4.424
Authors: Mark L Cutress; Grant D Stewart; Simon Wells-Cole; Simon Phipps; Ben G Thomas; David A Tolley Journal: BJU Int Date: 2012-05-07 Impact factor: 5.588
Authors: Eisuke Ueshima; Masashi Fujimori; Hiroshi Kodama; Diane Felsen; Jie Chen; Jeremy C Durack; Stephen B Solomon; Jonathan A Coleman; Govindarajan Srimathveeravalli Journal: Am J Physiol Renal Physiol Date: 2019-04-24