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Literature DB >> 2836078

Identification of a lys2 mutant of Candida maltosa by means of transformation.

G Kunze¹, R Bode, H Schmidt, I A Samsonova, D Birnbaum.

Abstract

We have isolated five mutants of Candida maltos, which lack the 2-aminoadipate reductase activity, an enzyme involved in the lysine biosynthesis. By means of complementation analysis using protoplast fusion, the isolated mutants were divided into two complementation groups. Thereof the C. maltosa strain G457 could be transformed by the plasmids pDP12 and pDP13, which contain the LYS2-coding gene of Saccharomyces cerevisiae. On the basis of our presented results obtained by studies on hybridization, stability, and recovery of plasmids from C. maltosa transformants, we suggest that transformation does proceed integratively.

Entities: Chemical Disease Species

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Year: 1987 PMID： 2836078 DOI： 10.1007/BF00378181

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

21 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. [Rhodosporidium Banno: dose-effect relations, mutagenic efficiency, and spectrum of mutants in the induction of auxotrophic mutants by ultraviolet light and N-methyl-N'-nitro-N-nitrosoguanidine].

Authors: F Böttcher; I A Samsonova
Journal: Z Allg Mikrobiol Date: 1978

3. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

4. Transformation of Rhodosporidium toruloides.

Authors: M Tully; H J Gilbert
Journal: Gene Date: 1985 Impact factor: 3.688

5. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.

Authors: G Simchen; F Winston; C A Styles; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

6. Preferential integration of yeast transposable element Ty into a promoter region.

Authors: H Eibel; P Philippsen
Journal: Nature Date: 1984 Jan 26-Feb 1 Impact factor: 49.962

7. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.

Authors: A C Chinault; J Carbon
Journal: Gene Date: 1979-02 Impact factor: 3.688

5. Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans.

Authors: R C Garrad; J K Bhattacharjee
Journal: J Bacteriol Date: 1992-11 Impact factor: 3.490

6. Physical and biochemical characterization of the cloned LYS5 gene required for alpha-aminoadipate reductase activity in the lysine biosynthetic pathway of Saccharomyces cerevisiae.

Authors: S Rajnarayan; J C Vaughn; J K Bhattacharjee
Journal: Curr Genet Date: 1992-01 Impact factor: 3.886

6 in total

Identification of a lys2 mutant of Candida maltosa by means of transformation.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. [Rhodosporidium Banno: dose-effect relations, mutagenic efficiency, and spectrum of mutants in the induction of auxotrophic mutants by ultraviolet light and N-methyl-N'-nitro-N-nitrosoguanidine].

3. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

4. Transformation of Rhodosporidium toruloides.

5. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.

6. Preferential integration of yeast transposable element Ty into a promoter region.

7. Overlap hybridization screening: isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome III.

Review 8. alpha-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes.

9. Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genome.

10. Studies on transformation of Escherichia coli with plasmids.

1. Cloning and characterization of a TEF gene for elongation factor 1 alpha from the yeast Arxula adeninivorans.

2. Characterization of Arxula adeninivorans strains from different habitats.

3. Cloning and physical characterization of linked lysine genes (lys4, lys15) of Saccharomyces cerevisiae.

4. A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements.

5. Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans.

6. Physical and biochemical characterization of the cloned LYS5 gene required for alpha-aminoadipate reductase activity in the lysine biosynthetic pathway of Saccharomyces cerevisiae.