Literature DB >> 15175929

A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements.

Yaroslav Terentiev1, Almudena Huarto Pico, Erik Böer, Thomas Wartmann, Jens Klabunde, Uta Breuer, Wolfgang Babel, Manfred Suckow, Gerd Gellissen, Gotthard Kunze.   

Abstract

An Arxula adeninivorans integration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1 promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level.

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Year:  2004        PMID： 15175929     DOI： 10.1007/s10295-004-0142-9

Source DB:  PubMed          Journal:  J Ind Microbiol Biotechnol        ISSN： 1367-5435            Impact factor:   3.346


  34 in total

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Journal:  Curr Genet       Date:  1994-07       Impact factor: 3.886

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  12 in total

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