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Literature DB >> 2834694

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.

V Knott¹, D J Rees, Z Cheng, G G Brownlee.

Abstract

Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.

Entities: Gene Species

Mesh：

Substances：

Year: 1988 PMID： 2834694 PMCID： PMC336392 DOI： 10.1093/nar/16.6.2601

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Toward a physical map of the genome of the nematode Caenorhabditis elegans.

Authors: A Coulson; J Sulston; S Brenner; J Karn
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

2. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors: D T Burke; G F Carle; M V Olson
Journal: Science Date: 1987-05-15 Impact factor: 47.728

3. Random-clone strategy for genomic restriction mapping in yeast.

Authors: M V Olson; J E Dutchik; M Y Graham; G M Brodeur; C Helms; M Frank; M MacCollin; R Scheinman; T Frank
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

4. A physical map of the Escherichia coli K12 genome.

Authors: C L Smith; J G Econome; A Schutt; S Klco; C R Cantor
Journal: Science Date: 1987-06-12 Impact factor: 47.728

5. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors: W B Wood
Journal: J Mol Biol Date: 1966-03 Impact factor: 5.469

6. A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion.

Authors: B G Herrmann; D P Barlow; H Lehrach
Journal: Cell Date: 1987-03-13 Impact factor: 41.582

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

8. Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter.

Authors: J E Larsen; K Gerdes; J Light; S Molin
Journal: Gene Date: 1984-04 Impact factor: 3.688

9. The construction of cosmid libraries which can be used to transform eukaryotic cells.

Authors: F G Grosveld; T Lund; E J Murray; A L Mellor; H H Dahl; R A Flavell
Journal: Nucleic Acids Res Date: 1982-11-11 Impact factor: 16.971

10. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.

Authors: J E Walker; N J Gay; M Saraste; A N Eberle
Journal: Biochem J Date: 1984-12-15 Impact factor: 3.857

22 in total

1. Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110.

Authors: R P Birkenbihl; W Vielmetter
Journal: Nucleic Acids Res Date: 1989-07-11 Impact factor: 16.971

2. Identification and characterization of a gene responsible for inhibiting propagation of methylated DNA sequences in mcrA mcrB1 Escherichia coli strains.

Authors: P L Kretz; S W Kohler; J M Short
Journal: J Bacteriol Date: 1991-08 Impact factor: 3.490

10. Generation of varicella-zoster virus (VZV) and viral mutants from cosmid DNAs: VZV thymidylate synthetase is not essential for replication in vitro.

Authors: J I Cohen; K E Seidel
Journal: Proc Natl Acad Sci U S A Date: 1993-08-01 Impact factor: 11.205

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome.

1. Toward a physical map of the genome of the nematode Caenorhabditis elegans.

2. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

3. Random-clone strategy for genomic restriction mapping in yeast.

4. A physical map of the Escherichia coli K12 genome.

5. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

6. A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion.

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

8. Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter.

9. The construction of cosmid libraries which can be used to transform eukaryotic cells.

10. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS.

1. Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110.

2. Identification and characterization of a gene responsible for inhibiting propagation of methylated DNA sequences in mcrA mcrB1 Escherichia coli strains.

3. Recombinant Epstein-Barr virus with small RNA (EBER) genes deleted transforms lymphocytes and replicates in vitro.

Review 4. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 5. Linkage map of Escherichia coli K-12, edition 8.

6. Completion of the detailed restriction map of the E. coli genome by the isolation of overlapping cosmid clones.

7. Molecular genetic analysis of Epstein-Barr virus Cp promoter function.

8. FISH detection of trisomy 21 in interphase by the simultaneous use of two differentially labelled cosmid contigs.

9. Epstein-Barr virus recombinants from overlapping cosmid fragments.

10. Generation of varicella-zoster virus (VZV) and viral mutants from cosmid DNAs: VZV thymidylate synthetase is not essential for replication in vitro.