Literature DB >> 6188729

Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating.

W M Huang, S J Gibson, P Facer, J Gu, J M Polak.   

Abstract

Poly-L-lysine (PPL) has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining. The adhesive properties of different molecular weight (m.w.) polymers of L-lysine have been tested on pre-fixed cryostat sections which were subjected to a 3 day washing treatment. It has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides. PLL (m.w. 350,000) at concentrations in the range of 0.05-0.1% was found to be the most effective polymer.

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Year:  1983        PMID： 6188729     DOI： 10.1007/bf00506570

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  6 in total

1.  Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons.

Authors:  T Hökfelt; J O Kellerth; G Nilsson; B Pernow
Journal:  Brain Res       Date:  1975-12-19       Impact factor: 3.252

2.  Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure.

Authors:  J Gu; K N Islam; J M Polak
Journal:  Histochem J       Date:  1983-05

3.  Use of polylysine-coated slides in preparation of cell samples for diagnostic cytology with special reference to urine sample.

Authors:  O A Husain; J A Millett; J M Grainger
Journal:  J Clin Pathol       Date:  1980-03       Impact factor: 3.411

4.  Insolubilization of protein antigens on polyacrylic plastic beads using poly-L-lysine.

Authors:  K Pachmann; W Leibold
Journal:  J Immunol Methods       Date:  1976       Impact factor: 2.303

5.  The distribution of nine peptides in rat spinal cord with special emphasis on the substantia gelatinosa and on the area around the central canal (lamina X).

Authors:  S J Gibson; J M Polak; S R Bloom; P D Wall
Journal:  J Comp Neurol       Date:  1981-09-01       Impact factor: 3.215

6.  Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy.

Authors:  D Mazia; G Schatten; W Sale
Journal:  J Cell Biol       Date:  1975-07       Impact factor: 10.539
  6 in total
  91 in total

1.  Osteoblast-specific gene expression after transplantation of marrow cells: implications for skeletal gene therapy.

Authors:  Z Hou; Q Nguyen; B Frenkel; S K Nilsson; M Milne; A J van Wijnen; J L Stein; P Quesenberry; J B Lian; G S Stein
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-22       Impact factor: 11.205

2.  In situ hybridisation of EBV DNA-DNA hybrids using wet heat in polypropylene containers.

Authors:  L G Labrecque
Journal:  J Clin Pathol       Date:  1992-12       Impact factor: 3.411

3.  Immunoreactive neuropeptide nerves in ligamentous tissue in chronic shoulder pain.

Authors:  Y T Konttinen; S Santavirta; P Paavolainen; I Antti-Poika; T Tiainen; D Nordström; M Hukkanen
Journal:  Arch Orthop Trauma Surg       Date:  1992       Impact factor: 3.067

4.  Improved method for bacterial cell capture after flow cytometry cell sorting.

Authors:  D Guillebault; M Laghdass; P Catala; I Obernosterer; P Lebaron
Journal:  Appl Environ Microbiol       Date:  2010-09-03       Impact factor: 4.792

5.  Early neuronal development in the spinal cord of a reptile assessed by neurofilament protein immunoreactivity.

Authors:  E Marti; M A Batista; A R Bello; A Lancha; D Dahl
Journal:  J Anat       Date:  1990-12       Impact factor: 2.610

6.  Association of viral 126 kDa protein-containing X-bodies with nuclei in mosaic-diseased tobacco leaves.

Authors:  M M Wijdeveld; R W Goldbach; B J Verduin; L C van Loon
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

7.  Peptide YY (PYY) immunoreactivity is co-stored with glucagon-related immunoreactants in endocrine cells of the gut and pancreas.

Authors:  A Ali-Rachedi; I M Varndell; T E Adrian; D A Gapp; S Van Noorden; S R Bloom; J M Polak
Journal:  Histochemistry       Date:  1984

8.  Bombesin-like immunoreactivity in the pancreas of man and other mammalian species.

Authors:  M A Ghatei; S K George; J H Major; F Carlei; J M Polak; S R Bloom
Journal:  Experientia       Date:  1984-08-15

9.  Occurrence, distribution and nature of neuropeptide Y in the rat pancreas.

Authors:  F Carlei; J M Allen; A E Bishop; S R Bloom; J M Polak
Journal:  Experientia       Date:  1985-12-15

10.  Immunocytochemical double staining of cytokeratin and prostate specific antigen in individual prostatic tumour cells.

Authors:  R Riesenberg; R Oberneder; M Kriegmair; M Epp; U Bitzer; A Hofstetter; S Braun; G Riethmüller; K Pantel
Journal:  Histochemistry       Date:  1993-01
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