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Literature DB >> 2830488

Repair of UV-induced lesions in Xenopus laevis oocytes.

R J Legerski¹, J E Penkala, C A Peterson, D A Wright.

Abstract

We characterized a DNA repair system in frog oocytes by comicroinjection of UV-irradiated pBR322 DNA and radiolabeled nucleotides. Repair synthesis was monitored by incorporation of label into recovered pBR322 DNA and by a novel method in which the removal of UV photoproducts was determined from the shift of DNA topoisomers that occurs during gel electrophoresis upon repair of these lesions. We investigated the effects of several drugs in the oocyte system and found that although novobiocin, an inhibitor of topoisomerase II, was an effective inhibitor of repair, VM-26, another inhibitor of topoisomerase II, was not. In addition, the topoisomerase I inhibitor camptothecin had no effect on repair in this system. Finally, circular DNA (either supercoiled or nicked circular) was repaired at least 50 times more rapidly than linear DNA.

Entities: Chemical Species

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Year: 1987 PMID： 2830488 PMCID： PMC368114 DOI： 10.1128/mcb.7.12.4317-4323.1987

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

30 in total

1. DNA synthesis in ocytes and eggs of Xenopus laevis injected with DNA.

Authors: C C Ford; H R Woodland
Journal: Dev Biol Date: 1975-03 Impact factor: 3.582

2. Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells.

Authors: K Mortelmans; E C Friedberg; H Slor; G Thomas; J E Cleaver
Journal: Proc Natl Acad Sci U S A Date: 1976-08 Impact factor: 11.205

3. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors: M Shure; D E Pulleyblank; J Vinograd
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

4. Detection and repair of single-strand breaks in nuclear DNA.

Authors: P R Cook; I A Brazell
Journal: Nature Date: 1976-10-21 Impact factor: 49.962

5. A sensitive endonuclease probe for lesions in deoxyribonucleic acid helix structure produced by carcinogenic or mutagenic agents.

Authors: R J Legerski; H B Gray; D L Robberson
Journal: J Biol Chem Date: 1977-12-10 Impact factor: 5.157

6. Comparison of the effect of ultraviolet radiation and ethidium bromide intercalation on the conformation of superhelical phiX174 replicative form DNA.

Authors: D T Denhardt; A C Kato
Journal: J Mol Biol Date: 1973-07-15 Impact factor: 5.469

Repair of UV-induced lesions in Xenopus laevis oocytes.

1. DNA synthesis in ocytes and eggs of Xenopus laevis injected with DNA.

2. Defective thymine dimer excision by cell-free extracts of xeroderma pigmentosum cells.

3. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

4. Detection and repair of single-strand breaks in nuclear DNA.

5. A sensitive endonuclease probe for lesions in deoxyribonucleic acid helix structure produced by carcinogenic or mutagenic agents.

6. Comparison of the effect of ultraviolet radiation and ethidium bromide intercalation on the conformation of superhelical phiX174 replicative form DNA.

7. The writhing number of a space curve.

8. Novobiocin does not inhibit DNA repair in an active gene.

9. Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.

10. Linear DNA does not form chromatin containing regularly spaced nucleosomes.

1. Repair and recombination of X-irradiated plasmids in Xenopus laevis oocytes.

2. Processing of targeted psoralen cross-links in Xenopus oocytes.

3. Excision repair of UV-damaged plasmid DNA in Xenopus oocytes is mediated by DNA polymerase alpha (and/or delta).

4. Composite transposable elements in the Xenopus laevis genome.

5. Repair of a synthetic abasic site in DNA in a Xenopus laevis oocyte extract.

6. Assembly of DNA polymerase delta and epsilon holoenzymes depends on the geometry of the DNA template.

7. DNA ligase I from Xenopus laevis eggs.

8. Identification and characterization of transcriptional arrest sites in exon 1 of the human adenosine deaminase gene.

9. Minichromosome assembly accompanying repair-type DNA synthesis in Xenopus oocytes.

10. Rapid and apparently error-prone excision repair of nonreplicating UV-irradiated plasmids in Xenopus laevis oocytes.