Literature DB >> 28298452

Applications of Digital PCR for Clinical Microbiology.

Jane Kuypers1, Keith R Jerome2.   

Abstract

Digital PCR (dPCR) is an important new tool for use in the clinical microbiology laboratory. Its advantages over quantitative PCR (qPCR), including absolute quantification without a standard curve, improved precision, improved accuracy in the presence of inhibitors, and more accurate quantitation when amplification efficiency is low, make dPCR the assay of choice for several specimen testing applications. This minireview will discuss the advantages and disadvantages of dPCR compared to qPCR, its applications in clinical microbiology, and considerations for implementation of the method in a clinical laboratory.
Copyright © 2017 American Society for Microbiology.

Keywords:  applications; digital PCR; quantitative PCR

Mesh：

Year:  2017        PMID： 28298452      PMCID： PMC5442518          DOI： 10.1128/JCM.00211-17

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  43 in total

1.  Next generation digital PCR measurement of hepatitis B virus copy number in formalin-fixed paraffin-embedded hepatocellular carcinoma tissue.

Authors:  Jing-Tao Huang; Ying-Juan Liu; Jin Wang; Zhi-Gao Xu; Ying Yang; Fan Shen; Xing-Hui Liu; Xin Zhou; Song-Mei Liu
Journal:  Clin Chem       Date:  2014-10-31       Impact factor: 8.327

2.  A short history of the polymerase chain reaction.

Authors:  John M S Bartlett; David Stirling
Journal:  Methods Mol Biol       Date:  2003

Review 3.  The potential advantages of digital PCR for clinical virology diagnostics.

Authors:  Ruth Hall Sedlak; Keith R Jerome
Journal:  Expert Rev Mol Diagn       Date:  2014-04-11       Impact factor: 5.225

4.  Marked variability of BK virus load measurement using quantitative real-time PCR among commonly used assays.

Authors:  Noah G Hoffman; Linda Cook; Ederlyn E Atienza; Ajit P Limaye; Keith R Jerome
Journal:  J Clin Microbiol       Date:  2008-06-18       Impact factor: 5.948

5.  Utility of droplet digital PCR for the quantitative detection of polyomavirus JC in clinical samples.

Authors:  Irene Giovannelli; Nunziata Ciccone; Guendalina Vaggelli; Nunzia Della Malva; Francesca Torricelli; Gian Maria Rossolini; Simone Giannecchini
Journal:  J Clin Virol       Date:  2016-07-18       Impact factor: 3.168

6.  Quantitative Detection and Genotyping of Helicobacter pylori from Stool using Droplet Digital PCR Reveals Variation in Bacterial Loads that Correlates with cagA Virulence Gene Carriage.

Authors:  Sarah Talarico; Mahboobeh Safaeian; Paula Gonzalez; Allan Hildesheim; Rolando Herrero; Carolina Porras; Bernal Cortes; Ann Larson; Ferric C Fang; Nina R Salama
Journal:  Helicobacter       Date:  2015-12-15       Impact factor: 5.753

Review 7.  Viral diagnostics in the era of digital polymerase chain reaction.

Authors:  Ruth Hall Sedlak; Keith R Jerome
Journal:  Diagn Microbiol Infect Dis       Date:  2012-11-20       Impact factor: 2.803

8.  Quantification of plasmid DNA reference materials for Shiga toxin-producing Escherichia coli based on UV, HR-ICP-MS and digital PCR.

Authors:  Wen Liang; Li Xu; Zhiwei Sui; Yan Li; Lanying Li; Yanli Wen; Chunhua Li; Shuzhen Ren; Gang Liu
Journal:  Chem Cent J       Date:  2016-09-12       Impact factor: 4.215

9.  Quantification of HEV RNA by Droplet Digital PCR.

Authors:  Florence Nicot; Michelle Cazabat; Sébastien Lhomme; Olivier Marion; Karine Sauné; Julie Chiabrando; Martine Dubois; Nassim Kamar; Florence Abravanel; Jacques Izopet
Journal:  Viruses       Date:  2016-08-19       Impact factor: 5.048

10.  Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Authors:  Alexandra S Whale; Claire A Bushell; Paul R Grant; Simon Cowen; Ion Gutierrez-Aguirre; Denise M O'Sullivan; Jana Žel; Mojca Milavec; Carole A Foy; Eleni Nastouli; Jeremy A Garson; Jim F Huggett
Journal:  J Clin Microbiol       Date:  2015-12-09       Impact factor: 5.948
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  45 in total

Review 1.  Slip-driven microfluidic devices for nucleic acid analysis.

Authors:  Weiyuan Lyu; Mengchao Yu; Haijun Qu; Ziqing Yu; Wenbin Du; Feng Shen
Journal:  Biomicrofluidics       Date:  2019-07-12       Impact factor: 2.800

2.  Highly Sensitive Detection of Isoniazid Heteroresistance in Mycobacterium tuberculosis by DeepMelt Assay.

Authors:  Bin Liang; Yaoju Tan; Zi Li; Xueshan Tian; Chen Du; Hui Li; Guoli Li; Xiangyang Yao; Zhongan Wang; Ye Xu; Qingge Li
Journal:  J Clin Microbiol       Date:  2018-01-24       Impact factor: 5.948

3.  Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.

Authors:  April Aralar; Yixu Yuan; Kevin Chen; Yunshu Geng; Daniel Ortiz Velez; Mridu Sinha; Shelley M Lawrence; Stephanie I Fraley
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

4.  Comparison of Three Adenovirus Quantitative PCR Assays with ATCC Reference Strains and Clinical Samples.

Authors:  Kimberly Starr; Alexander L Greninger; Negar Makhsous; Keith R Jerome; Linda Cook
Journal:  J Clin Microbiol       Date:  2019-10-23       Impact factor: 5.948

Review 5.  Forty Years of Molecular Diagnostics for Infectious Diseases.

Authors:  Jonathan E Schmitz; Charles W Stratton; David H Persing; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2022-07-19       Impact factor: 11.677

6.  Development and Application of a Duplex Droplet Digital Polymerase Chain Reaction Assay for Detection and Differentiation of EP402R-Deleted and Wild-Type African Swine Fever Virus.

Authors:  Junhai Zhu; Weijun Jian; Yifan Huang; Qi Gao; Fei Gao; Huahan Chen; Guihong Zhang; Ming Liao; Wenbao Qi
Journal:  Front Vet Sci       Date:  2022-06-06

7.  Evaluation of changes in intestinal microbiota in Crohn's disease patients after anti-TNF alpha treatment.

Authors:  Laura Sanchis-Artero; Juan Francisco Martínez-Blanch; Sergio Manresa-Vera; Ernesto Cortés-Castell; Marina Valls-Gandia; Marisa Iborra; Jose Maria Paredes-Arquiola; Maia Boscá-Watts; Jose Maria Huguet; Rafael Gil-Borrás; Josefa Rodríguez-Morales; Xavier Cortés-Rizo
Journal:  Sci Rep       Date:  2021-05-11       Impact factor: 4.379

8.  An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2.

Authors:  Tim Boogaerts; Lotte Jacobs; Naomi De Roeck; Siel Van den Bogaert; Bert Aertgeerts; Lies Lahousse; Alexander L N van Nuijs; Peter Delputte
Journal:  Sci Total Environ       Date:  2021-05-26       Impact factor: 7.963

9.  SARS-CoV-2 RNAemia Predicts Clinical Deterioration and Extrapulmonary Complications from COVID-19.

Authors:  Nikhil Ram-Mohan; David Kim; Elizabeth J Zudock; Marjan M Hashemi; Kristel C Tjandra; Angela J Rogers; Catherine A Blish; Kari C Nadeau; Jennifer A Newberry; James V Quinn; Ruth O'Hara; Euan Ashley; Hien Nguyen; Lingxia Jiang; Paul Hung; Andra L Blomkalns; Samuel Yang
Journal:  Clin Infect Dis       Date:  2022-01-29       Impact factor: 9.079

10.  Correlating qRT-PCR, dPCR and Viral Titration for the Identification and Quantification of SARS-CoV-2: A New Approach for Infection Management.

Authors:  Martina Brandolini; Francesca Taddei; Maria Michela Marino; Laura Grumiro; Agata Scalcione; Maria Elena Turba; Fabio Gentilini; Michela Fantini; Silvia Zannoli; Giorgio Dirani; Vittorio Sambri
Journal:  Viruses       Date:  2021-05-28       Impact factor: 5.048
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