Literature DB >> 24724628

The potential advantages of digital PCR for clinical virology diagnostics.

Ruth Hall Sedlak1, Keith R Jerome.   

Abstract

Digital PCR (dPCR), a new nucleic acid amplification technology, offers several potential advantages over real-time or quantitative PCR (qPCR), the current workhorse of clinical molecular virology diagnostics. Several studies have demonstrated dPCR assays for human cytomegalovirus or HIV, which give more precise and reproducible results than qPCR assays without sacrificing sensitivity. Here we review the literature comparing dPCR and qPCR performance in viral molecular diagnostic assays and offer perspective on the future of dPCR in clinical virology diagnostics.

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Year:  2014        PMID： 24724628     DOI： 10.1586/14737159.2014.910456

Source DB:  PubMed          Journal:  Expert Rev Mol Diagn        ISSN： 1473-7159            Impact factor:   5.225


  18 in total

Review 1.  Applications of Digital PCR for Clinical Microbiology.

Authors:  Jane Kuypers; Keith R Jerome
Journal:  J Clin Microbiol       Date:  2017-03-15       Impact factor: 5.948

Review 2.  The Future of Digital Polymerase Chain Reaction in Virology.

Authors:  Matthijs Vynck; Wim Trypsteen; Olivier Thas; Linos Vandekerckhove; Ward De Spiegelaere
Journal:  Mol Diagn Ther       Date:  2016-10       Impact factor: 4.074

3.  Superiority of Digital Reverse Transcription-PCR (RT-PCR) over Real-Time RT-PCR for Quantitation of Highly Divergent Human Rhinoviruses.

Authors:  Ruth Hall Sedlak; Thuy Nguyen; Isabel Palileo; Keith R Jerome; Jane Kuypers
Journal:  J Clin Microbiol       Date:  2016-11-23       Impact factor: 5.948

Review 4.  Past, present, and future perspectives on the diagnosis of Roseolovirus infections.

Authors:  Joshua A Hill; Ruth Hall Sedlak; Keith R Jerome
Journal:  Curr Opin Virol       Date:  2014-10-14       Impact factor: 7.090

5.  Quantification and Trends of Rotavirus and Enterovirus in Untreated Sewage Using Reverse Transcription Droplet Digital PCR.

Authors:  Nicholas M Kiulia; Raul Gonzalez; Hannah Thompson; Tiong Gim Aw; Joan B Rose
Journal:  Food Environ Virol       Date:  2021-02-16       Impact factor: 2.778

6.  Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison.

Authors:  Reza Nouri; Ming Dong; Anthony J Politza; Weihua Guan
Journal:  ACS Sens       Date:  2022-03-03       Impact factor: 9.618

7.  Digital PCR for Detection and Quantification of Fluoroquinolone Resistance in Legionella pneumophila.

Authors:  Aurélie Hennebique; Marie Bidart; Sophie Jarraud; Laëtitia Beraud; Carole Schwebel; Max Maurin; Sandrine Boisset
Journal:  Antimicrob Agents Chemother       Date:  2017-08-24       Impact factor: 5.191

8.  DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials.

Authors:  Philippe Corbisier; Leonardo Pinheiro; Stéphane Mazoua; Anne-Marie Kortekaas; Pui Yan Jenny Chung; Tsvetelina Gerganova; Gert Roebben; Hendrik Emons; Kerry Emslie
Journal:  Anal Bioanal Chem       Date:  2015-01-20       Impact factor: 4.142

9.  Absolute measurement of gene transcripts with Selfie-digital PCR.

Authors:  Petar Podlesniy; Ramon Trullas
Journal:  Sci Rep       Date:  2017-08-21       Impact factor: 4.379

10.  Validation of a digital PCR method for quantification of DNA copy number concentrations by using a certified reference material.

Authors:  Liesbet Deprez; Philippe Corbisier; Anne-Marie Kortekaas; Stéphane Mazoua; Roxana Beaz Hidalgo; Stefanie Trapmann; Hendrik Emons
Journal:  Biomol Detect Quantif       Date:  2016-08-30
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