| Literature DB >> 28288123 |
Wei Zhang1, Alexander W Lohman2,3, Yevgeniya Zhuravlova1, Xiaocen Lu1, Matthew D Wiens1, Hiofan Hoi1, Sine Yaganoglu4, Manuel A Mohr4, Elena N Kitova1,5, John S Klassen1,5, Periklis Pantazis4, Roger J Thompson2,3, Robert E Campbell1.
Abstract
To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.Entities:
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Year: 2017 PMID： 28288123 DOI： 10.1038/nmeth.4222
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547