| Literature DB >> 28280149 |
Weimin Zhang1,2, Guanghou Zhao1,3, Zhouqing Luo1, Yicong Lin1, Lihui Wang1, Yakun Guo1, Ann Wang1, Shuangying Jiang1, Qingwen Jiang1, Jianhui Gong4, Yun Wang4, Sha Hou1, Jing Huang1, Tianyi Li1,2, Yiran Qin1, Junkai Dong1, Qin Qin1, Jiaying Zhang1,2, Xinzhi Zou1, Xi He1, Li Zhao1, Yibo Xiao1, Meng Xu1, Erchao Cheng1, Ning Huang1, Tong Zhou1, Yue Shen4,5,6, Roy Walker5, Yisha Luo5, Zheng Kuang7, Leslie A Mitchell7, Kun Yang8, Sarah M Richardson8, Yi Wu9, Bing-Zhi Li9, Ying-Jin Yuan9, Huanming Yang4,10, Jiwei Lin11, Guo-Qiang Chen1, Qingyu Wu1, Joel S Bader8, Yizhi Cai5, Jef D Boeke7, Junbiao Dai12.
Abstract
We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in Saccharomyces cerevisiae SynXII was assembled using a two-step method, specified by successive megachunk integration and meiotic recombination-mediated assembly, producing a functional chromosome in S. cerevisiae. Minor growth defect "bugs" detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a Saccharomyces synXII strain that would be identified as Saccharomyces bayanus by standard DNA barcoding procedures.Entities:
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Year: 2017 PMID： 28280149 DOI： 10.1126/science.aaf3981
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728