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Literature DB >> 2827168

Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition.

Abstract

Short DNA sequences at ends of transposable elements are needed as sites for transposition. Previous deletion mapping showed that, in Tn5 and its component IS50 elements, these essential sites are about 19 base pairs long. To determine which positions are important in transposition, we made one or more sequence changes at each position in the IS50 outside (O) end and assayed the effects of these changes on transposition. Our results indicate that the specific base pairs at 18 of the 19 positions are important in transposition. A 9-base-pair segment in the O end corresponds to a binding site for the Escherichia coli DnaA protein. Comparisons of effects of mutations at different positions in this site, and also measurements of Tn5 transposition in dnaA- cells, indicate that DnaA protein participates in O-end-mediated transposition.

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Year: 1987 PMID： 2827168 PMCID： PMC299703 DOI： 10.1073/pnas.84.24.9118

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

22 in total

1. Sequences essential for IS50 transposition. The first base-pair.

Authors: C Sasakawa; S H Phadnis; G F Carle; D E Berg
Journal: J Mol Biol Date: 1985-04-20 Impact factor: 5.469

2. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

Authors: W I Wood; J Gitschier; L A Lasky; R M Lawn
Journal: Proc Natl Acad Sci U S A Date: 1985-03 Impact factor: 11.205

3. DNA structure. Bent molecules--how and why?

Authors: D Lilley
Journal: Nature Date: 1986 Apr 10-16 Impact factor: 49.962

4. Minimal size plasmids containing an M13 origin for production of single-strand transducing particles.

Authors: A Levinson; D Silver; B Seed
Journal: J Mol Appl Genet Date: 1984

5. Structural requirement for IS50-mediated gene transposition.

Authors: D E Berg
Journal: Proc Natl Acad Sci U S A Date: 1983-02 Impact factor: 11.205

6. IS50-mediated inverse transposition. Discrimination between the two ends of an IS element.

Authors: C Sasakawa; D E Berg
Journal: J Mol Biol Date: 1982-08-05 Impact factor: 5.469

7. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors: E Y Chen; P H Seeburg
Journal: DNA Date: 1985-04

8. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.

Authors: T A Baker; K Sekimizu; B E Funnell; A Kornberg
Journal: Cell Date: 1986-04-11 Impact factor: 41.582

9. Iron(II) EDTA used to measure the helical twist along any DNA molecule.

Authors: T D Tullius; B A Dombroski
Journal: Science Date: 1985-11-08 Impact factor: 47.728

10. DNA sequences at the ends of transposon Tn5 required for transposition.

Authors: R C Johnson; W S Reznikoff
Journal: Nature Date: 1983 Jul 21-27 Impact factor: 49.962

23 in total

1. Functional analysis of the 3'-terminal sequence of the maize controlling element (Ac) by internal replacement and deletion mutagenesis.

Authors: J H Zhou; A Myers; A G Atherly
Journal: Genetica Date: 1991 Impact factor: 1.082

6. Identification and purification of a Drosophila protein that binds to the terminal 31-base-pair inverted repeats of the P transposable element.

Authors: D C Rio; G M Rubin
Journal: Proc Natl Acad Sci U S A Date: 1988-12 Impact factor: 11.205

Identification of base pairs in the outside end of insertion sequence IS50 that are needed for IS50 and Tn5 transposition.

1. Sequences essential for IS50 transposition. The first base-pair.

2. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

3. DNA structure. Bent molecules--how and why?

4. Minimal size plasmids containing an M13 origin for production of single-strand transducing particles.

5. Structural requirement for IS50-mediated gene transposition.

6. IS50-mediated inverse transposition. Discrimination between the two ends of an IS element.

7. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

8. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.

9. Iron(II) EDTA used to measure the helical twist along any DNA molecule.

10. DNA sequences at the ends of transposon Tn5 required for transposition.

1. Functional analysis of the 3'-terminal sequence of the maize controlling element (Ac) by internal replacement and deletion mutagenesis.

2. Intramolecular transposition by a synthetic IS50 (Tn5) derivative.

3. Site-specific transposition of insertion sequence IS630.

4. Integration host factor plays a role in IS50 and Tn5 transposition.

5. Effects of variation of inverted-repeat sequences on reactions mediated by the transposase of Tn21.

6. Identification and purification of a Drosophila protein that binds to the terminal 31-base-pair inverted repeats of the P transposable element.

7. Mutational analysis of insertion sequence 50 (IS50) and transposon 5 (Tn5) ends.

8. The organization of the outside end of transposon Tn5.

9. Tn5 insertion specificity is not influenced by IS50 end sequences in target DNA.

10. Binding sites for maize nuclear proteins in the terminal inverted repeats of the Mu1 transposable element.