| Literature DB >> 2989531 |
C Sasakawa, S H Phadnis, G F Carle, D E Berg.
Abstract
Sequences near the ends of the insertion element IS50 are essential for its transposition, probably because they serve as sites upon which the IS50-encoded transposase protein acts. To determine if these essential sequences include the first base-pair at each end of IS50 we generated 5'C to 5'G transversions at these positions. Each mutation reduced the transposition frequency to 1% to 2% of wild-type. DNA sequence analyses showed that the mutant 5'G is preserved during transposition.Mesh：
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Year: 1985 PMID： 2989531 DOI： 10.1016/0022-2836(85)90235-9
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469