Literature DB >> 28261707

Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits.

Seungyong You1, James Froberg1, Junru Yu2, Manas Haldar3, Abbas Sedigh2, Sanku Mallik3, D K Srivastava2, Yongki Choi1.   

Abstract

Using single-molecule approaches, we directly observed the dynamic interaction between HDAC8 and various ligands as well as conformational interconversions during the catalytic reaction. Statistical analysis identified key kinetic parameters, demonstrating that the enzymatic activity is highly sensitive to both minor variations in the ligand structures and small synthetic molecules.

Entities:  

Mesh：

Substances：

Year:  2017        PMID： 28261707      PMCID： PMC5510773          DOI： 10.1039/c6cc09949a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  26 in total

1.  A 21st century revisionist's view at a turning point in enzymology.

Authors:  Zachary D Nagel; Judith P Klinman
Journal:  Nat Chem Biol       Date:  2009-08       Impact factor: 15.040

2.  Single-molecule lysozyme dynamics monitored by an electronic circuit.

Authors:  Yongki Choi; Issa S Moody; Patrick C Sims; Steven R Hunt; Brad L Corso; Israel Perez; Gregory A Weiss; Philip G Collins
Journal:  Science       Date:  2012-01-20       Impact factor: 47.728

3.  Histone deacetylase activators: N-acetylthioureas serve as highly potent and isozyme selective activators for human histone deacetylase-8 on a fluorescent substrate.

Authors:  Raushan K Singh; Tanmay Mandal; Narayanaganesh Balsubramanian; Tajae Viaene; Travis Leedahl; Nitesh Sule; Gregory Cook; D K Srivastava
Journal:  Bioorg Med Chem Lett       Date:  2011-08-04       Impact factor: 2.823

4.  Coumarin-suberoylanilide hydroxamic acid as a fluorescent probe for determining binding affinities and off-rates of histone deacetylase inhibitors.

Authors:  Raushan K Singh; Tanmay Mandal; Narayanaganesh Balasubramanian; Gregory Cook; D K Srivastava
Journal:  Anal Biochem       Date:  2010-09-22       Impact factor: 3.365

5.  Single-molecule dynamics of lysozyme processing distinguishes linear and cross-linked peptidoglycan substrates.

Authors:  Yongki Choi; Issa S Moody; Patrick C Sims; Steven R Hunt; Brad L Corso; David E Seitz; Larry C Blaszczak; Larry C Blaszcazk; Philip G Collins; Gregory A Weiss
Journal:  J Am Chem Soc       Date:  2012-01-24       Impact factor: 15.419

6.  Variable active site loop conformations accommodate the binding of macrocyclic largazole analogues to HDAC8.

Authors:  Christophe Decroos; Dane J Clausen; Brandon E Haines; Olaf Wiest; Robert M Williams; David W Christianson
Journal:  Biochemistry       Date:  2015-03-20       Impact factor: 3.162

7.  A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.

Authors:  Gira Bhabha; Jeeyeon Lee; Damian C Ekiert; Jongsik Gam; Ian A Wilson; H Jane Dyson; Stephen J Benkovic; Peter E Wright
Journal:  Science       Date:  2011-04-08       Impact factor: 47.728

8.  Structural snapshots of human HDAC8 provide insights into the class I histone deacetylases.

Authors:  John R Somoza; Robert J Skene; Bradley A Katz; Clifford Mol; Joseph D Ho; Andy J Jennings; Christine Luong; Andrew Arvai; Joseph J Buggy; Ellen Chi; Jie Tang; Bi-Ching Sang; Erik Verner; Robert Wynands; Ellen M Leahy; Douglas R Dougan; Gyorgy Snell; Marc Navre; Mark W Knuth; Ronald V Swanson; Duncan E McRee; Leslie W Tari
Journal:  Structure       Date:  2004-07       Impact factor: 5.006

9.  Crystal structure of a eukaryotic zinc-dependent histone deacetylase, human HDAC8, complexed with a hydroxamic acid inhibitor.

Authors:  Alessandro Vannini; Cinzia Volpari; Gessica Filocamo; Elena Caroli Casavola; Mirko Brunetti; Debora Renzoni; Prasun Chakravarty; Chantal Paolini; Raffaele De Francesco; Paola Gallinari; Christian Steinkühler; Stefania Di Marco
Journal:  Proc Natl Acad Sci U S A       Date:  2004-10-11       Impact factor: 11.205

10.  Loop interactions and dynamics tune the enzymatic activity of the human histone deacetylase 8.

Authors:  Micha B A Kunze; David W Wright; Nicolas D Werbeck; John Kirkpatrick; Peter V Coveney; D Flemming Hansen
Journal:  J Am Chem Soc       Date:  2013-11-15       Impact factor: 15.419
View more
  1 in total

1.  Real-time tracking of single-molecule collagenase on native collagen and partially structured collagen-mimic substrates.

Authors:  James Froberg; Woo-Sik Choi; Abbas Sedigh; Tayebeh Anajafi; Jasmin Farmakes; Zhongyu Yang; Sanku Mallik; D K Srivastava; Yongki Choi
Journal:  Chem Commun (Camb)       Date:  2018-09-11       Impact factor: 6.222
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.