Literature DB >> 2826131

The interaction of the recognition helix of lac repressor with lac operator.

N Lehming1, J Sartorius, M Niemöller, G Genenger, B v Wilcken-Bergmann, B Müller-Hill.   

Abstract

We have constructed a system which allows systematic testing of repressor--operator interactions. The system consists of two plasmids. One of them carries a lac operon in which lac operator has been replaced by a unique restriction site into which synthetic operators can be cloned. The other plasmid carries the gene coding for the repressor, in our case a semisynthetic lacI gene of which parts can be exchanged in a cassette-like manner. A galE host allows us to select for mutants which express repressors with altered specificities. Here we report the change of specificity in the lac system by changing residues 1 and 2 of the recognition helix of lac repressor. The specificity changes are brought about cooperatively by the change of both residues. Exchanges of just one residue broaden the specificity. Our results hint that the recognition helix of lac repressor may possibly have the opposite orientation to those in Lambda cro protein or 434 CI repressor.

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Year:  1987        PMID： 2826131      PMCID： PMC553756          DOI： 10.1002/j.1460-2075.1987.tb02625.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  35 in total

1.  Genetic regulatory mechanisms in the synthesis of proteins.

Authors:  F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1961-06       Impact factor: 5.469

2.  Specific destruction of the second lac operator decreases repression of the lac operon in Escherichia coli fivefold.

Authors:  E Eismann; B von Wilcken-Bergmann; B Müller-Hill
Journal:  J Mol Biol       Date:  1987-06-20       Impact factor: 5.469

3.  Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance.

Authors:  R Boelens; R M Scheek; J H van Boom; R Kaptein
Journal:  J Mol Biol       Date:  1987-01-05       Impact factor: 5.469

4.  How lac repressor binds to DNA.

Authors:  K Adler; K Beyreuther; E Fanning; N Geisler; B Gronenborn; A Klemm; B Müller-Hill; M Pfahl; A Schmitz
Journal:  Nature       Date:  1972-06-09       Impact factor: 49.962

5.  Structure of the DNA-binding region of lac repressor inferred from its homology with cro repressor.

Authors:  B W Matthews; D H Ohlendorf; W F Anderson; Y Takeda
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

6.  Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors:  E Y Chen; P H Seeburg
Journal:  DNA       Date:  1985-04

7.  Model of specific complex between catabolite gene activator protein and B-DNA suggested by electrostatic complementarity.

Authors:  I T Weber; T A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  General selection for specific DNA-binding activities.

Authors:  N Benson; P Sugiono; S Bass; L V Mendelman; P Youderian
Journal:  Genetics       Date:  1986-09       Impact factor: 4.562

9.  Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.

Authors:  R H Ebright; P Cossart; B Gicquel-Sanzey; J Beckwith
Journal:  Nature       Date:  1984 Sep 20-26       Impact factor: 49.962

10.  Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter.

Authors:  M Besse; B von Wilcken-Bergmann; B Müller-Hill
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598
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  45 in total

1.  Strong DNA binding by covalently linked dimeric Lac headpiece: evidence for the crucial role of the hinge helices.

Authors:  C G Kalodimos; G E Folkers; R Boelens; R Kaptein
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-15       Impact factor: 11.205

2.  A functional assay in Escherichia coli to detect non-assisted interaction between galactose repressor dimers.

Authors:  N Perez; M Rehault; M Amouyal
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

3.  Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.

Authors:  Charalampos G Kalodimos; Alexandre M J J Bonvin; Roberto K Salinas; Rainer Wechselberger; Rolf Boelens; Robert Kaptein
Journal:  EMBO J       Date:  2002-06-17       Impact factor: 11.598

4.  Specificities of three tight-binding Lac repressors.

Authors:  P Kolkhof
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

5.  Successive binding of raf repressor to adjacent raf operator sites in vitro.

Authors:  C Aslanidis; I Muiznieks; R Schmitt
Journal:  Mol Gen Genet       Date:  1990-09

6.  Mutations that define the optimal half-site for binding yeast GCN4 activator protein and identify an ATF/CREB-like repressor that recognizes similar DNA sites.

Authors:  J W Sellers; A C Vincent; K Struhl
Journal:  Mol Cell Biol       Date:  1990-10       Impact factor: 4.272

7.  cDNA from rat cells with reconstitutive galactose-epimerase activity in E. coli.

Authors:  M Zeschnigk; B von Wilcken-Bergmann; A Starzinski-Powitz
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

8.  Functional rules for lac repressor-operator associations and implications for protein-DNA interactions.

Authors:  Leslie Milk; Robert Daber; Mitchell Lewis
Journal:  Protein Sci       Date:  2010-06       Impact factor: 6.725

9.  "Cold-sensitive" mutants of the Lac repressor.

Authors:  Andrew Barker; Stefan Oehler; Benno Müller-Hill
Journal:  J Bacteriol       Date:  2006-12-15       Impact factor: 3.490

10.  Mutations in the bZIP domain of yeast GCN4 that alter DNA-binding specificity.

Authors:  D Tzamarias; W T Pu; K Struhl
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205
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