| Literature DB >> 3116268 |
E Eismann1, B von Wilcken-Bergmann, B Müller-Hill.
Abstract
The second operator of the lac operon, located within the 5'-coding region of the lacZ gene, was specifically destroyed by means of oligonucleotide-directed mutagenesis. Eight of its bases were exchanged without altering the wild-type amino acid sequence of beta-galactosidase. The mutation was transferred onto an F'lac+I+O+Z+pro+ episome. We observed a fivefold decrease in repression of beta-galactosidase expression compared to that in the wild-type.Entities:
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Year: 1987 PMID： 3116268 DOI： 10.1016/0022-2836(87)90499-2
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469