Literature DB >> 17172344

"Cold-sensitive" mutants of the Lac repressor.

Andrew Barker1, Stefan Oehler, Benno Müller-Hill.   

Abstract

Thirteen of more than 4,000 single-amino-acid-replacement mutants of the Lac repressor, generated by suppression of amber nonsense mutants, were characterized as having a cold-sensitive phenotype. However, when expressed as missense mutations, none of the replacements cause cold sensitivity, implicating the suppression mechanism as being responsible for this phenotype.

Mesh：

Substances：

Year:  2006        PMID： 17172344      PMCID： PMC1855763          DOI： 10.1128/JB.01462-06

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  Dimerisation mutants of Lac repressor. I. A monomeric mutant, L251A, that binds Lac operator DNA as a dimer.

Authors:  F Dong; S Spott; O Zimmermann; B Kisters-Woike; B Müller-Hill; A Barker
Journal:  J Mol Biol       Date:  1999-07-16       Impact factor: 5.469

2.  Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR.

Authors:  Milko B Kermekchiev; Anatoly Tzekov; Wayne M Barnes
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

3.  Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.

Authors:  Charalampos G Kalodimos; Alexandre M J J Bonvin; Roberto K Salinas; Rainer Wechselberger; Rolf Boelens; Robert Kaptein
Journal:  EMBO J       Date:  2002-06-17       Impact factor: 11.598

4.  Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences.

Authors:  N Lehming; J Sartorius; S Oehler; B von Wilcken-Bergmann; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

5.  Dimerisation mutants of Lac repressor. II. A single amino acid substitution, D278L, changes the specificity of dimerisation.

Authors:  S Spott; F Dong; B Kisters-Woike; B Müller-Hill
Journal:  J Mol Biol       Date:  2000-02-18       Impact factor: 5.469

6.  The three operators of the lac operon cooperate in repression.

Authors:  S Oehler; E R Eismann; H Krämer; B Müller-Hill
Journal:  EMBO J       Date:  1990-04       Impact factor: 11.598

7.  Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli.

Authors:  Brian Søgaard Laursen; Igor Siwanowicz; Guilhem Larigauderie; Jakob Hedegaard; Koreaki Ito; Yoshikazu Nakamura; John M Kenney; Kim Kusk Mortensen; Hans Uffe Sperling-Petersen
Journal:  J Mol Biol       Date:  2003-02-14       Impact factor: 5.469

8.  Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.

Authors:  P Markiewicz; L G Kleina; C Cruz; S Ehret; J H Miller
Journal:  J Mol Biol       Date:  1994-07-29       Impact factor: 5.469

9.  Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.

Authors:  M Lewis; G Chang; N C Horton; M A Kercher; H C Pace; M A Schumacher; R G Brennan; P Lu
Journal:  Science       Date:  1996-03-01       Impact factor: 47.728

10.  The interaction of the recognition helix of lac repressor with lac operator.

Authors:  N Lehming; J Sartorius; M Niemöller; G Genenger; B v Wilcken-Bergmann; B Müller-Hill
Journal:  EMBO J       Date:  1987-10       Impact factor: 11.598
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.