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Literature DB >> 2823228

Plasmid migration using orthogonal-field-alternation gel electrophoresis.

Abstract

The migration properties of a series of supercoiled plasmids ranging in size from 4 to 16 kilobases (kb) have been analyzed by orthogonal-field-alternation gel electrophoresis (OFAGE). These circular DNAs enter the gel and are well resolved. Unlike linear DNA molecules, the relative mobilities of these plasmids are constant over a wide range of pulse times, from 10 to 120 seconds, as well as over a broad range of total running times, from 6 to 24 hours. Electrophoresis of supercoiled, relaxed, and nicked open circular forms as well as topoisomers of pBR322 shows that the extent of supercoiling has a dramatic effect on plasmid migration on OFAGE. Several practical applications for exploiting the different migration properties of circular and linear DNA molecules on OFAGE are presented.

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Year: 1987 PMID： 2823228 PMCID： PMC306366 DOI： 10.1093/nar/15.20.8387

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

19 in total

1. Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.

Authors: S Mickel; V Arena; W Bauer
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

2. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

Authors: P H Johnson; L I Grossman
Journal: Biochemistry Date: 1977-09-20 Impact factor: 3.162

3. The gel electrophoresis of DNA.

Authors: C Aaij; P Borst
Journal: Biochim Biophys Acta Date: 1972-05-10

Review 4. DNA topoisomerases.

Authors: M Gellert
Journal: Annu Rev Biochem Date: 1981 Impact factor: 23.643

5. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors: G F Carle; M V Olson
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

6. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

7. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

Authors: C L Smith; T Matsumoto; O Niwa; S Klco; J B Fan; M Yanagida; C R Cantor
Journal: Nucleic Acids Res Date: 1987-06-11 Impact factor: 16.971

8. An electrophoretic karyotype for yeast.

Authors: G F Carle; M V Olson
Journal: Proc Natl Acad Sci U S A Date: 1985-06 Impact factor: 11.205

9. Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum.

Authors: D J Kemp; L M Corcoran; R L Coppel; H D Stahl; A E Bianco; G V Brown; R F Anders
Journal: Nature Date: 1985 May 23-29 Impact factor: 49.962

10. Chromosomes of kinetoplastida.

Authors: L H Van der Ploeg; A W Cornelissen; J D Barry; P Borst
Journal: EMBO J Date: 1984-12-20 Impact factor: 11.598

29 in total

1. A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus.

Authors: T H Tzeng; L K Lyngholm; C F Ford; C R Bronson
Journal: Genetics Date: 1992-01 Impact factor: 4.562

9. Pulsed field gel electrophoresis reveals chromosome length differences between strains of Cladosporium fulvum (syn. Fulvia fulva).

Authors: N J Talbot; R P Oliver; A Coddington
Journal: Mol Gen Genet Date: 1991-10

10. Amplified DNAs in laboratory stocks of Leishmania tarentolae: extrachromosomal circles structurally and functionally similar to the inverted-H-region amplification of methotrexate-resistant Leishmania major.

Authors: M L Petrillo-Peixoto; S M Beverley
Journal: Mol Cell Biol Date: 1988-12 Impact factor: 4.272

Plasmid migration using orthogonal-field-alternation gel electrophoresis.

1. Physical properties and gel electrophoresis behavior of R12-derived plasmid DNAs.

2. Electrophoresis of DNA in agarose gels. Optimizing separations of conformational isomers of double- and single-stranded DNAs.

3. The gel electrophoresis of DNA.

Review 4. DNA topoisomerases.

5. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

6. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

7. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

8. An electrophoretic karyotype for yeast.

9. Size variation in chromosomes from independent cultured isolates of Plasmodium falciparum.

10. Chromosomes of kinetoplastida.

1. A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus.

2. Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus.

Review 3. New approaches for physical mapping of small genomes.

4. 2 microns plasmid multimers in the YAC host strain AB1380 interfere with some applications of YAC libraries.

5. Genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834.

6. Characterization of the 'unusual' mobility of large circular DNAs in pulsed field-gradient electrophoresis.

7. Pulsed-field gel electrophoresis of circular DNA.

8. Investigating DNA migration in pulsed fields using a miniaturized FIGE system.

9. Pulsed field gel electrophoresis reveals chromosome length differences between strains of Cladosporium fulvum (syn. Fulvia fulva).

10. Amplified DNAs in laboratory stocks of Leishmania tarentolae: extrachromosomal circles structurally and functionally similar to the inverted-H-region amplification of methotrexate-resistant Leishmania major.