Literature DB >> 281704

Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line.

J H Nunberg, R J Kaufman, R T Schimke, G Urlaub, L A Chasin.   

Abstract

Methotrexate-resistant Chinese hamster ovary cells selected for high resistance by progressive increments of methotrexate in the culture medium have levels of dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate: NADP+ oxidoreductase, EC 1.5.1.3) 200 times that of sensitive cells and a corresponding increase in the number of copies of the dihydrofolate reductase gene. The resistant cells contain an expanded region on the second chromosome (homogeneously staining region) that is not present in sensitive cells. In situ hybridization of DNA complementary to dihydrofolate reductase mRNA shows that the dihydrofolate reductase genes are specifically localized to the homogeneously staining region of this chromosome in the resistant cells.

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Year:  1978        PMID： 281704      PMCID： PMC393004          DOI： 10.1073/pnas.75.11.5553

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Nucleic acid hybridization to the DNA of cytological preparations.

Authors:  M L Pardue; J G Gall
Journal:  Methods Cell Biol       Date:  1975       Impact factor: 1.441

2.  CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.

Authors:  R G HAM
Journal:  Proc Natl Acad Sci U S A       Date:  1965-02       Impact factor: 11.205

3.  Synthesis and degradation of folate reductase in sensitive and methotrexate-resistant lines of S-180 cells.

Authors:  F W Alt; R E Kellems; R T Schimke
Journal:  J Biol Chem       Date:  1976-05-25       Impact factor: 5.157

4.  Amplification of dihydrofolate reductase genes in methotrexate-resistant cultured mouse cells.

Authors:  R T Schimke; F W Alt; R E Kellems; R J Kaufman; J R Bertino
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

5.  Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

Authors:  A C Chang; J H Nunberg; R J Kaufman; H A Erlich; R T Schimke; S N Cohen
Journal:  Nature       Date:  1978-10-19       Impact factor: 49.962

Review 6.  Tandem genetic duplications in phage and bacteria.

Authors:  R P Anderson; J R Roth
Journal:  Annu Rev Microbiol       Date:  1977       Impact factor: 15.500

7.  Mutations affecting adenine phosphoribosyl transferase activity in Chinese hamster cells.

Authors:  L A Chasin
Journal:  Cell       Date:  1974-05       Impact factor: 41.582

8.  A rapid banding technique for human chromosomes.

Authors:  M Seabright
Journal:  Lancet       Date:  1971-10-30       Impact factor: 79.321

9.  Quantitation of dihydrofolate reductase in individual parental and methotrexate-resistant murine cells. Use of a fluorescence activated cell sorter.

Authors:  R J Kaufman; J R Bertino; R T Schimke
Journal:  J Biol Chem       Date:  1978-08-25       Impact factor: 5.157

10.  Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors:  F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157
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  116 in total

Review 1.  Resistance to cytostatic drugs at the cellular level.

Authors:  C P Vendrik; J J Bergers; W H De Jong; P A Steerenberg
Journal:  Cancer Chemother Pharmacol       Date:  1992       Impact factor: 3.333

2.  Activation of a mammalian origin of replication by chromosomal rearrangement.

Authors:  T H Leu; J L Hamlin
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272

Review 3.  Double minutes, cytogenetic equivalents of gene amplification, in human neoplasia - a review.

Authors:  Erich Gebhart
Journal:  Clin Transl Oncol       Date:  2005-12       Impact factor: 3.405

4.  Cytogenetical changes during early stages of development of methotrexate resistance in HeLa cells.

Authors:  J G Delinassios; M J Talieri
Journal:  Med Oncol Tumor Pharmacother       Date:  1985

5.  Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.

Authors:  R J Kaufman; L C Wasley; A J Spiliotes; S D Gossels; S A Latt; G R Larsen; R M Kay
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272

6.  Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.

Authors:  J Brennand; A C Chinault; D S Konecki; D W Melton; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

7.  MDM2 regulates dihydrofolate reductase activity through monoubiquitination.

Authors:  Maria Maguire; Paul C Nield; Timothy Devling; Rosalind E Jenkins; B Kevin Park; Radoslaw Polański; Nikolina Vlatković; Mark T Boyd
Journal:  Cancer Res       Date:  2008-05-01       Impact factor: 12.701

8.  Quantitative correlation of homogeneously stained regions on chromosome 10 with dihydrofolate reductase enzyme in human cells.

Authors:  S R Wolman; M L Craven; S P Grill; B A Domin; Y C Cheng
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

9.  Specific DNA sequence amplification in human neuroblastoma cells.

Authors:  K T Montgomery; J L Biedler; B A Spengler; P W Melera
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

10.  Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene.

Authors:  W F Flintoff; E Livingston; C Duff; R G Worton
Journal:  Mol Cell Biol       Date:  1984-01       Impact factor: 4.272
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