| Literature DB >> 28142079 |
Jinhwa Lee1, Hai Yu2, Yonghai Li1, Jingjiao Ma1, Yuekun Lang1, Michael Duff1, Jamie Henningson1, Qinfang Liu2, Yuhao Li1, Abdou Nagy1, Bhupinder Bawa1, Zejun Li3, Guangzhi Tong3, Juergen A Richt1, Wenjun Ma4.
Abstract
Although several studies have investigated the functions of influenza PA-X, the impact of different expressions of PA-X protein including full-length, truncated or PA-X deficient forms on virus replication, pathogenicity and host response remains unclear. Herein, we generated two mutated viruses expressing a full-length or deficient PA-X protein based on the A/California/04/2009 (H1N1) virus that expresses a truncated PA-X to understand three different expressions of PA-X protein on virus replication, pathogenicity and host immune responses. The results showed that expression of either full-length or truncated PA-X protein enhanced viral replication and pathogenicity as well as reduced host innate immune response in mice by host shutoff activity when compared to the virus expressing the deficient PA-X form. Furthermore, the full-length PA-X expression exhibited a greater effect on virus pathogenicity than the truncated PA-X form. Our results provide novel insights of PA-X on viral replication, pathogenicity and host immune responses.Entities:
Keywords: Host immune response; Influenza A virus; PA-X; Virus replication
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Year: 2017 PMID： 28142079 PMCID： PMC5651993 DOI： 10.1016/j.virol.2017.01.015
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616