Literature DB >> 2813059

Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid.

H Tanimura1, M Maeda, T Fukazawa, M Sekine, T Hata.   

Abstract

A general and practical synthetic method of oligoribonucleotides (10-20 mers) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl (Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5'-hydroxyls and tetrahydropyranyl (Thp) group was used for the 2'-hydroxyl protecting groups. In addition, suitable acyl groups were introduced for the protection of the lactam functions of guanine and uracil moieties.

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Year:  1989        PMID： 2813059      PMCID： PMC334953          DOI： 10.1093/nar/17.20.8135

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Polymer-supported RNA synthesis and its application to test the nearest-neighbor model for duplex stability.

Authors:  R Kierzek; M H Caruthers; C E Longfellow; D Swinton; D H Turner; S M Freier
Journal:  Biochemistry       Date:  1986-12-02       Impact factor: 3.162

2.  Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethoxycarbonyl (Fmoc) for 5'-hydroxyl protection.

Authors:  C Lehmann; Y Z Xu; C Christodoulou; Z K Tan; M J Gait
Journal:  Nucleic Acids Res       Date:  1989-04-11       Impact factor: 16.971

3.  Potato spindle tuber "virus". IV. A replicating, low molecular weight RNA.

Authors:  T O Diener
Journal:  Virology       Date:  1971-08       Impact factor: 3.616

4.  The effect of acceptor oligoribonucleotide sequence on the T4 RNA ligase reaction.

Authors:  E Romaniuk; L W McLaughlin; T Neilson; P J Romaniuk
Journal:  Eur J Biochem       Date:  1982-07

5.  5'-Levulinyl and 2'-tetrahydrofuranyl protection for the synthesis of oligoribonucleotides by the phosphoramidite approach.

Authors:  S Iwai; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

6.  Reversal of T4 RNA ligase.

Authors:  M Krug; O C Uhlenbeck
Journal:  Biochemistry       Date:  1982-04-13       Impact factor: 3.162

7.  Mapping adenines, guanines, and pyrimidines in RNA.

Authors:  H Donis-Keller; A M Maxam; W Gilbert
Journal:  Nucleic Acids Res       Date:  1977-08       Impact factor: 16.971

8.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

9.  A new solid-phase synthesis of oligoribonucleotides by the phosphoro-p-anisidate method using tetrahydrofuranyl protection of 2'-hydroxyl groups.

Authors:  S Iwai; E Yamada; M Asaka; Y Hayase; H Inoue; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1987-05-11       Impact factor: 16.971

10.  Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy.

Authors:  T Ohno; N Takamatsu; T Meshi; Y Okada
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971
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  1 in total

1.  Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Authors:  R Iwase; M Maeda; T Fujiwara; M Sekine; T Hata; K Miura
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971
  1 in total

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