| Literature DB >> 28111461 |
Sergey Shmakov1,2, Aaron Smargon3,4, David Scott3, David Cox3, Neena Pyzocha3,5, Winston Yan3, Omar O Abudayyeh3,6, Jonathan S Gootenberg3,7, Kira S Makarova2, Yuri I Wolf2, Konstantin Severinov1,8,9, Feng Zhang3,6,7,10,11, Eugene V Koonin2.
Abstract
Class 2 CRISPR-Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRISPR-Cas subtypes. The diverse properties of these new systems provide potential for the development of versatile tools for genome editing and regulation. In this Analysis article, we present a comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes, outline evolutionary scenarios for the independent origin of different class 2 CRISPR-Cas systems from mobile genetic elements, and propose an amended classification and nomenclature of CRISPR-Cas.Entities:
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Year: 2017 PMID： 28111461 PMCID： PMC5851899 DOI： 10.1038/nrmicro.2016.184
Source DB: PubMed Journal: Nat Rev Microbiol ISSN： 1740-1526 Impact factor: 60.633