Literature DB >> 28103687

Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe.

Wei Qiang Ong1, Y Rose Citron2, Sayaka Sekine1, Bo Huang1.   

Abstract

Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

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Year:  2016        PMID： 28103687      PMCID： PMC5460623          DOI： 10.1021/acschembio.6b00586

Source DB:  PubMed          Journal:  ACS Chem Biol        ISSN： 1554-8929            Impact factor:   5.100


  36 in total

1.  Visualizing the distribution and transport of mRNAs in living cells.

Authors:  Diana P Bratu; Byeong-Jik Cha; Musa M Mhlanga; Fred Russell Kramer; Sanjay Tyagi
Journal:  Proc Natl Acad Sci U S A       Date:  2003-10-28       Impact factor: 11.205

2.  Universal aptamer-based real-time monitoring of enzymatic RNA synthesis.

Authors:  Katharina Höfer; Lisa V Langejürgen; Andres Jäschke
Journal:  J Am Chem Soc       Date:  2013-09-04       Impact factor: 15.419

Review 3.  Structure and Mechanism of RNA Mimics of Green Fluorescent Protein.

Authors:  Mingxu You; Samie R Jaffrey
Journal:  Annu Rev Biophys       Date:  2015       Impact factor: 12.981

4.  Translation-independent localization of mRNA in E. coli.

Authors:  Keren Nevo-Dinur; Anat Nussbaum-Shochat; Sigal Ben-Yehuda; Orna Amster-Choder
Journal:  Science       Date:  2011-02-25       Impact factor: 47.728

5.  Flow cytometric detection of specific RNAs in native human cells with quenched autoligating FRET probes.

Authors:  Hiroshi Abe; Eric T Kool
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-29       Impact factor: 11.205

Review 6.  mRNA localization: gene expression in the spatial dimension.

Authors:  Kelsey C Martin; Anne Ephrussi
Journal:  Cell       Date:  2009-02-20       Impact factor: 41.582

7.  Programmable RNA Tracking in Live Cells with CRISPR/Cas9.

Authors:  David A Nelles; Mark Y Fang; Mitchell R O'Connell; Jia L Xu; Sebastian J Markmiller; Jennifer A Doudna; Gene W Yeo
Journal:  Cell       Date:  2016-03-17       Impact factor: 41.582

8.  Asymmetric mRNA localization contributes to fidelity and sensitivity of spatially localized systems.

Authors:  Robert J Weatheritt; Toby J Gibson; M Madan Babu
Journal:  Nat Struct Mol Biol       Date:  2014-08-24       Impact factor: 15.369

9.  Single-cell systems biology by super-resolution imaging and combinatorial labeling.

Authors:  Eric Lubeck; Long Cai
Journal:  Nat Methods       Date:  2012-06-03       Impact factor: 28.547

10.  Background free imaging of single mRNAs in live cells using split fluorescent proteins.

Authors:  Bin Wu; Jiahao Chen; Robert H Singer
Journal:  Sci Rep       Date:  2014-01-09       Impact factor: 4.379
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  11 in total

Review 1.  Recent progress in single-molecule studies of mRNA localization in vivo.

Authors:  Songhee H Kim; Melissa Vieira; Jae Youn Shim; Hongyoung Choi; Hye Yoon Park
Journal:  RNA Biol       Date:  2018-11-14       Impact factor: 4.652

2.  Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments.

Authors:  Rinat Arbel-Goren; Yonatan Shapira; Joel Stavans
Journal:  J Vis Exp       Date:  2017-12-21       Impact factor: 1.355

Review 3.  Nucleic-Acid Structures as Intracellular Probes for Live Cells.

Authors:  Devleena Samanta; Sasha B Ebrahimi; Chad A Mirkin
Journal:  Adv Mater       Date:  2019-07-04       Impact factor: 30.849

Review 4.  RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs.

Authors:  Yichi Su; Ming C Hammond
Journal:  Curr Opin Biotechnol       Date:  2020-02-19       Impact factor: 9.740

5.  RNA-based fluorescent biosensors for live cell detection of bacterial sRNA.

Authors:  Rebekah Z Kitto; Kylee E Christiansen; Ming C Hammond
Journal:  Biopolymers       Date:  2020-08-12       Impact factor: 2.505

Review 6.  The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research.

Authors:  Saumya Das; K Mark Ansel; Markus Bitzer; Xandra O Breakefield; Alain Charest; David J Galas; Mark B Gerstein; Mihir Gupta; Aleksandar Milosavljevic; Michael T McManus; Tushar Patel; Robert L Raffai; Joel Rozowsky; Matthew E Roth; Julie A Saugstad; Kendall Van Keuren-Jensen; Alissa M Weaver; Louise C Laurent
Journal:  Cell       Date:  2019-04-04       Impact factor: 66.850

Review 7.  Light-Up RNA Aptamers and Their Cognate Fluorogens: From Their Development to Their Applications.

Authors:  Farah Bouhedda; Alexis Autour; Michael Ryckelynck
Journal:  Int J Mol Sci       Date:  2017-12-23       Impact factor: 5.923

8.  Crystal structure and fluorescence properties of the iSpinach aptamer in complex with DFHBI.

Authors:  Pablo Fernandez-Millan; Alexis Autour; Eric Ennifar; Eric Westhof; Michael Ryckelynck
Journal:  RNA       Date:  2017-09-22       Impact factor: 4.942

Review 9.  Making the Message Clear: Concepts for mRNA Imaging.

Authors:  Kristina Rau; Andrea Rentmeister
Journal:  ACS Cent Sci       Date:  2017-07-06       Impact factor: 14.553

10.  Programmable RNA detection with a fluorescent RNA aptamer using optimized three-way junction formation.

Authors:  Yuichi Furuhata; Mizuki Kobayashi; Ryo Maruyama; Yusuke Sato; Kurumi Makino; Tatsuo Michiue; Hiroharu Yui; Seiichi Nishizawa; Keitaro Yoshimoto
Journal:  RNA       Date:  2019-02-11       Impact factor: 4.942
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