| Literature DB >> 28099840 |
Jun Ueda1, Akihito Harada2, Takashi Urahama3, Shinichi Machida3, Kazumitsu Maehara2, Masashi Hada4, Yoshinori Makino4, Jumpei Nogami2, Naoki Horikoshi3, Akihisa Osakabe3, Hiroyuki Taguchi3, Hiroki Tanaka3, Hiroaki Tachiwana3, Tatsuma Yao5, Minami Yamada6, Takashi Iwamoto6, Ayako Isotani7, Masahito Ikawa7, Taro Tachibana8, Yuki Okada4, Hiroshi Kimura9, Yasuyuki Ohkawa2, Hitoshi Kurumizaka3, Kazuo Yamagata10.
Abstract
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.Entities:
Keywords: chromatin; crystal structure; epigenetics; histone variant; meiosis; nucleosome; spermatogenesis; spermatogonial differentiation; spermatozoa; testes
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Year: 2017 PMID： 28099840 DOI： 10.1016/j.celrep.2016.12.065
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423