Pan Pan1, Chad W Skaer1, Hsin-Tzu Wang1, Michael A Kreiser1, Steven M Stirdivant2, Kiyoko Oshima3, Yi-Wen Huang4, Matthew R Young5, Li-Shu Wang1. 1. a Department of Medicine , Division of Hematology and Oncology, Medical College of Wisconsin , Milwaukee , Wisconsin , USA. 2. b Clinical Research and Development, Metabolon, Inc. , Durham , North Carolina , USA. 3. c Department of Pathology , Medical College of Wisconsin , Milwaukee , Wisconsin , USA. 4. d Department of Obstetrics and Gynecology , Medical College of Wisconsin , Milwaukee , Wisconsin , USA. 5. e Division of Cancer Prevention, National Cancer Institute , Rockville , Maryland , USA.
Abstract
INTRODUCTION: Freeze-dried black raspberries (BRBs) elicit chemopreventive effects against colorectal cancer in humans and in rodents. The objective of this study was to investigate potential BRB-caused metabolite changes using wild-type (WT) C57BL/6 mice. METHODS AND RESULTS: WT mice were fed either control diet or control diet supplemented with 5% BRBs for 8 wk. A nontargeted metabolomic analysis was conducted on colonic mucosa, liver, and fecal specimens collected from both diet groups. BRBs significantly changed the levels of 41 colonic mucosa metabolites, 40 liver metabolites, and 34 fecal metabolites compared to control diet-fed mice. BRBs reduced 34 lipid metabolites in colonic mucosa and increased levels of amino acids in liver. One metabolite, 3-[3-(sulfooxy) phenyl] propanoic acid, might be a useful biomarker of BRB consumption. In addition, BRB powder was found to contain 30-fold higher levels of linolenate compared to control diets. Consistently, multiple omega-3 polyunsaturated fatty acids (ω-3 PUFAs), including stearidonate, docosapentaenoate (ω-3 DPA), eicosapentaenoate (EPA), and docosahexaenoate (DHA), were significantly elevated in livers of BRB-fed mice. CONCLUSION: The data from the current study suggest that BRBs produce systemic metabolite changes in multiple tissue matrices, supporting our hypothesis that BRBs may serve as both a chemopreventive agent and a beneficial dietary supplement.
INTRODUCTION: Freeze-dried black raspberries (BRBs) elicit chemopreventive effects against colorectal cancer in humans and in rodents. The objective of this study was to investigate potential BRB-caused metabolite changes using wild-type (WT) C57BL/6 mice. METHODS AND RESULTS: WT mice were fed either control diet or control diet supplemented with 5% BRBs for 8 wk. A nontargeted metabolomic analysis was conducted on colonic mucosa, liver, and fecal specimens collected from both diet groups. BRBs significantly changed the levels of 41 colonic mucosa metabolites, 40 liver metabolites, and 34 fecal metabolites compared to control diet-fed mice. BRBs reduced 34 lipid metabolites in colonic mucosa and increased levels of amino acids in liver. One metabolite, 3-[3-(sulfooxy) phenyl] propanoic acid, might be a useful biomarker of BRB consumption. In addition, BRB powder was found to contain 30-fold higher levels of linolenate compared to control diets. Consistently, multiple omega-3 polyunsaturated fatty acids (ω-3 PUFAs), including stearidonate, docosapentaenoate (ω-3 DPA), eicosapentaenoate (EPA), and docosahexaenoate (DHA), were significantly elevated in livers of BRB-fed mice. CONCLUSION: The data from the current study suggest that BRBs produce systemic metabolite changes in multiple tissue matrices, supporting our hypothesis that BRBs may serve as both a chemopreventive agent and a beneficial dietary supplement.
Authors: Sandro J R Bonatto; Heloisa H P Oliveira; Everson A Nunes; Daniele Pequito; Fabiola Iagher; Isabela Coelho; Katya Naliwaiko; Marcelo Kryczyk; Gleisson A P Brito; João Repka; Luciano V Sabóia; George Fukujima; Philip C Calder; Luiz C Fernandes Journal: Lipids Date: 2011-12-11 Impact factor: 1.880
Authors: Michel Carlos Mocellin; Juliana de Aguiar Pastore e Silva; Carolina de Quadros Camargo; Maria Emília de Souza Fabre; Scheila Gevaerd; Katya Naliwaiko; Yara Maria Franco Moreno; Everson Araújo Nunes; Erasmo Benicio Santos de Moraes Trindade Journal: Lipids Date: 2013-07-26 Impact factor: 1.880
Authors: Pan Pan; Chad W Skaer; Steven M Stirdivant; Matthew R Young; Gary D Stoner; John F Lechner; Yi-Wen Huang; Li-Shu Wang Journal: Cancer Prev Res (Phila) Date: 2015-06-08
Authors: Karla Sánchez-Lara; Jenny G Turcott; Eva Juárez-Hernández; Carolina Nuñez-Valencia; Geraldine Villanueva; Patricia Guevara; Martha De la Torre-Vallejo; Alejandro Mohar; Oscar Arrieta Journal: Clin Nutr Date: 2014-04-04 Impact factor: 7.324