Pan Pan1, Daniel S Peiffer1,2, Yi-Wen Huang3, Kiyoko Oshima4,5, Gary D Stoner1, Li-Shu Wang1. 1. Department of Medicine, Division of Hematology and Oncology, Medical College of Wisconsin, Milwaukee, WI, USA. 2. Current: Stritch School of Medicine, Loyola University Chicago, Chicago, IL, USA. 3. Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI, USA. 4. Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA. 5. Current: Department of Pathology, John Hopkins University, Baltimore, MD, USA.
Abstract
BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is one of two subtypes of esophageal cancer, with high incidence and mortality rates in developing countries. OBJECTIVE: The current study investigated the potential chemoprotective effects of strawberries and aspirin against the development of rat esophageal papillomas, the precursors to ESCC. METHODS: Using a prevention model, we administered study diets to rats before, during, and after N-nitrosomethylbenzylamine (NMBA) treatment. The effects of the four diets were evaluated: the control diet, 5% strawberry powder in the control diet, 0.01% aspirin in the drinking water, and the combination of strawberries and aspirin. At week 25, we euthanized all the rats and collected their esophagi to quantify tumor incidence, multiplicity, and burden, as well as for molecular analysis. RESULTS: Both strawberries and aspirin significantly decreased esophageal tumor multiplicity, with the combination causing the most robust suppression. Aspirin alone and the combination decreased the total tumor burden in the esophagus. None of the diets had a significant effect on tumor incidence or the expression of COX-1 and COX-2. Strawberries and aspirin, alone and in combination, significantly suppressed squamous epithelial cell proliferation (PCNA). CONCLUSIONS: Strawberries, aspirin, and their combination exhibit chemoprotective effects against NMBA-induced esophageal tumors in rats.
BACKGROUND:Esophageal squamous cell carcinoma (ESCC) is one of two subtypes of esophageal cancer, with high incidence and mortality rates in developing countries. OBJECTIVE: The current study investigated the potential chemoprotective effects of strawberries and aspirin against the development of ratesophageal papillomas, the precursors to ESCC. METHODS: Using a prevention model, we administered study diets to rats before, during, and after N-nitrosomethylbenzylamine (NMBA) treatment. The effects of the four diets were evaluated: the control diet, 5% strawberry powder in the control diet, 0.01% aspirin in the drinking water, and the combination of strawberries and aspirin. At week 25, we euthanized all the rats and collected their esophagi to quantify tumor incidence, multiplicity, and burden, as well as for molecular analysis. RESULTS: Both strawberries and aspirin significantly decreased esophageal tumor multiplicity, with the combination causing the most robust suppression. Aspirin alone and the combination decreased the total tumor burden in the esophagus. None of the diets had a significant effect on tumor incidence or the expression of COX-1 and COX-2. Strawberries and aspirin, alone and in combination, significantly suppressed squamous epithelial cell proliferation (PCNA). CONCLUSIONS: Strawberries, aspirin, and their combination exhibit chemoprotective effects against NMBA-induced esophageal tumors in rats.
Authors: Li-Shu Wang; Chieh-Ti Kuo; Tim H-M Huang; Martha Yearsley; Kiyoko Oshima; Gary D Stoner; Jianhua Yu; John F Lechner; Yi-Wen Huang Journal: Cancer Prev Res (Phila) Date: 2013-10-15
Authors: Nancy N Zikri; Kenneth M Riedl; Li-Shu Wang; John Lechner; Steven J Schwartz; Gary D Stoner Journal: Nutr Cancer Date: 2009 Impact factor: 2.900
Authors: Marilie D Gammon; Mary Beth Terry; Nadir Arber; Wong-Ho Chow; Harvey A Risch; Thomas L Vaughan; Janet B Schoenberg; Susan T Mayne; Janet L Stanford; Robert Dubrow; Heidrun Rotterdam; A Brian West; Joseph F Fraumeni; I Bernard Weinstein; Hanina Hibshoosh Journal: Cancer Epidemiol Biomarkers Prev Date: 2004-01 Impact factor: 4.254
Authors: Colin Baigent; Lisa Blackwell; Rory Collins; Jonathan Emberson; Jon Godwin; Richard Peto; Julie Buring; Charles Hennekens; Patricia Kearney; Tom Meade; Carlo Patrono; Maria Carla Roncaglioni; Alberto Zanchetti Journal: Lancet Date: 2009-05-30 Impact factor: 79.321