Literature DB >> 28058688

Targeted mutagenesis of Drosophila RNaseZ gene by homologous recombination.

O V Andreenkov1, E I Volkova1, S A Demakov2, X Xie3, E B Dubrovsky3, I F Zhimulev1.   

Abstract

For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.

Entities:  

Mesh：

Substances：

Year:  2017        PMID： 28058688     DOI： 10.1134/S1607672916060065

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  9 in total

1.  ELAC2, a putative prostate cancer susceptibility gene product, potentiates TGF-beta/Smad-induced growth arrest of prostate cells.

Authors:  D Noda; S Itoh; Y Watanabe; M Inamitsu; S Dennler; F Itoh; S Koike; D Danielpour; P ten Dijke; M Kato
Journal:  Oncogene       Date:  2006-04-24       Impact factor: 9.867

2.  From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering.

Authors:  Juan Huang; Wenke Zhou; Wei Dong; Annie M Watson; Yang Hong
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-08       Impact factor: 11.205

3.  Efficient ends-out gene targeting in Drosophila.

Authors:  Juan Huang; Wenke Zhou; Annie M Watson; Yuh-Nung Jan; Yang Hong
Journal:  Genetics       Date:  2008-08-30       Impact factor: 4.562

4.  The isolation of two juvenile hormone-inducible genes in Drosophila melanogaster.

Authors:  E B Dubrovsky; V A Dubrovskaya; A L Bilderback; E M Berger
Journal:  Dev Biol       Date:  2000-08-15       Impact factor: 3.582

5.  RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues.

Authors:  Xie Xie; Veronica A Dubrovskaya; Edward B Dubrovsky
Journal:  Insect Biochem Mol Biol       Date:  2010-12-10       Impact factor: 4.714

6.  A candidate prostate cancer susceptibility gene at chromosome 17p.

Authors:  S V Tavtigian; J Simard; D H Teng; V Abtin; M Baumgard; A Beck; N J Camp; A R Carillo; Y Chen; P Dayananth; M Desrochers; M Dumont; J M Farnham; D Frank; C Frye; S Ghaffari; J S Gupte; R Hu; D Iliev; T Janecki; E N Kort; K E Laity; A Leavitt; G Leblanc; J McArthur-Morrison; A Pederson; B Penn; K T Peterson; J E Reid; S Richards; M Schroeder; R Smith; S C Snyder; B Swedlund; J Swensen; A Thomas; M Tranchant; A M Woodland; F Labrie; M H Skolnick; S Neuhausen; J Rommens; L A Cannon-Albright
Journal:  Nat Genet       Date:  2001-02       Impact factor: 38.330

7.  Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system.

Authors:  Xie Xie; Veronica Dubrovskaya; Nancy Yacoub; Joanna Walska; Tara Gleason; Katherine Reid; Edward B Dubrovsky
Journal:  Dev Biol       Date:  2013-07-15       Impact factor: 3.582

8.  Human cytosolic tRNase ZL can downregulate gene expression through miRNA.

Authors:  Reyad A Elbarbary; Hiroaki Takaku; Naoto Uchiumi; Hiroko Tamiya; Mayumi Abe; Hiroshi Nishida; Masayuki Nashimoto
Journal:  FEBS Lett       Date:  2009-09-12       Impact factor: 4.124

9.  Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo.

Authors:  Edward B Dubrovsky; Veronica A Dubrovskaya; Louis Levinger; Steffen Schiffer; Anita Marchfelder
Journal:  Nucleic Acids Res       Date:  2004-01-09       Impact factor: 16.971
  9 in total
  1 in total

1.  Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo.

Authors:  Aleksandra Pajak; Isabelle Laine; Paula Clemente; Najla El-Fissi; Florian A Schober; Camilla Maffezzini; Javier Calvo-Garrido; Rolf Wibom; Roberta Filograna; Ashish Dhir; Anna Wedell; Christoph Freyer; Anna Wredenberg
Journal:  PLoS Genet       Date:  2019-07-31       Impact factor: 5.917
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.