Literature DB >> 28005393

Multiplexed Single-Cell Imaging: Past, Present, and Future.

Jia-Ren Lin1.   

Abstract

Jia-Ren Lin from the Laboratory of Systems Pharmacology at Harvard Medical School was awarded best poster at the annual Society of Biomolecular Imaging and Informatics meeting held in Boston, September 2016. His work focuses on single-cell imaging, especially on developing new methods for simultaneously detecting many antigens, named cyclic immunofluorescence (CycIF). This method could be applied in different stages of drug development, from discovery phase, preclinical research to clinical research. The current works and future directions of CycIF method are summarized in the following overview.

Keywords:  cyclic immunofluorescence; drug discovery; multiplexed imaging

Mesh:

Year:  2016        PMID: 28005393      PMCID: PMC5248548          DOI: 10.1089/adt.2016.765

Source DB:  PubMed          Journal:  Assay Drug Dev Technol        ISSN: 1540-658X            Impact factor:   1.738


  11 in total

1.  Analyzing proteome topology and function by automated multidimensional fluorescence microscopy.

Authors:  Walter Schubert; Bernd Bonnekoh; Ansgar J Pommer; Lars Philipsen; Raik Böckelmann; Yanina Malykh; Harald Gollnick; Manuela Friedenberger; Marcus Bode; Andreas W M Dress
Journal:  Nat Biotechnol       Date:  2006-10-01       Impact factor: 54.908

2.  CLARITY for mapping the nervous system.

Authors:  Kwanghun Chung; Karl Deisseroth
Journal:  Nat Methods       Date:  2013-06       Impact factor: 28.547

3.  Highly multiplexed phenotypic imaging for cell proliferation studies.

Authors:  Paolo Cappella; Fabio Gasparri
Journal:  J Biomol Screen       Date:  2013-07-29

4.  Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development.

Authors:  Sean C Bendall; Kara L Davis; El-Ad David Amir; Michelle D Tadmor; Erin F Simonds; Tiffany J Chen; Daniel K Shenfeld; Garry P Nolan; Dana Pe'er
Journal:  Cell       Date:  2014-04-24       Impact factor: 41.582

5.  Optical imaging. Expansion microscopy.

Authors:  Fei Chen; Paul W Tillberg; Edward S Boyden
Journal:  Science       Date:  2015-01-15       Impact factor: 47.728

6.  Multiplexed ion beam imaging of human breast tumors.

Authors:  Michael Angelo; Sean C Bendall; Rachel Finck; Matthew B Hale; Chuck Hitzman; Alexander D Borowsky; Richard M Levenson; John B Lowe; Scot D Liu; Shuchun Zhao; Yasodha Natkunam; Garry P Nolan
Journal:  Nat Med       Date:  2014-03-02       Impact factor: 53.440

7.  Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.

Authors:  Michael J Gerdes; Christopher J Sevinsky; Anup Sood; Sudeshna Adak; Musodiq O Bello; Alexander Bordwell; Ali Can; Alex Corwin; Sean Dinn; Robert J Filkins; Denise Hollman; Vidya Kamath; Sireesha Kaanumalle; Kevin Kenny; Melinda Larsen; Michael Lazare; Qing Li; Christina Lowes; Colin C McCulloch; Elizabeth McDonough; Michael C Montalto; Zhengyu Pang; Jens Rittscher; Alberto Santamaria-Pang; Brion D Sarachan; Maximilian L Seel; Antti Seppo; Kashan Shaikh; Yunxia Sui; Jingyu Zhang; Fiona Ginty
Journal:  Proc Natl Acad Sci U S A       Date:  2013-07-01       Impact factor: 11.205

8.  Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems.

Authors:  Evan Murray; Jae Hun Cho; Daniel Goodwin; Taeyun Ku; Justin Swaney; Sung-Yon Kim; Heejin Choi; Young-Gyun Park; Jeong-Yoon Park; Austin Hubbert; Margaret McCue; Sara Vassallo; Naveed Bakh; Matthew P Frosch; Van J Wedeen; H Sebastian Seung; Kwanghun Chung
Journal:  Cell       Date:  2015-12-03       Impact factor: 41.582

9.  Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

Authors:  Jia-Ren Lin; Mohammad Fallahi-Sichani; Peter K Sorger
Journal:  Nat Commun       Date:  2015-09-24       Impact factor: 14.919

10.  Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.

Authors:  Daniel P Riordan; Sushama Varma; Robert B West; Patrick O Brown
Journal:  PLoS One       Date:  2015-07-15       Impact factor: 3.240

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  1 in total

1.  Unraveling mitotic protein networks by 3D multiplexed epitope drug screening.

Authors:  Lorenz J Maier; Stefan M Kallenberger; Katharina Jechow; Marcel Waschow; Roland Eils; Christian Conrad
Journal:  Mol Syst Biol       Date:  2018-08-13       Impact factor: 11.429

  1 in total

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