| Literature DB >> 27992828 |
Jienan Li1, Haibo Luo1, Feng Song1, Lushun Zhang2, Chuncao Deng1, Zailiang Yu3, Tianzhen Gao1, Miao Liao1, Yiping Hou4.
Abstract
Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.Entities:
Keywords: Forensic science; Microreader™ 23sp ID system; Short tandem repeat (STR); Validation
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Year: 2016 PMID： 27992828 DOI： 10.1016/j.fsigen.2016.12.005
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN： 1872-4973 Impact factor: 4.882