| Literature DB >> 27955870 |
Rosario Vivek1, Syed Fazil Ahamed1, Shalini Kotabagi1, Anmol Chandele2, Ira Khanna3, Navin Khanna3, Kaustuv Nayak2, Mary Dias4, Murali-Krishna Kaja5, Anita Shet6.
Abstract
The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.Entities:
Keywords: Dengue; Diagnostics; Evaluation; NS1 protein; Rapid diagnostic test
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Year: 2016 PMID： 27955870 DOI： 10.1016/j.diagmicrobio.2016.09.020
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803