Madhusudhan Bysani1, Alexander Perfilyev1, Vanessa D de Mello2, Tina Rönn1, Emma Nilsson1, Jussi Pihlajamäki2,3, Charlotte Ling1. 1. Epigenetics & Diabetes Unit, Department of Clinical Sciences, Lund University Diabetes Centre, Malmö, Sweden. 2. Department of Clinical Nutrition, Institute of Public Health & Clinical Nutrition, University of Eastern Finland, Kuopio, Finland. 3. Clinical Nutrition & Obesity Center, Kuopio University Hospital, Kuopio, Finland.
Abstract
AIM: To study the impact of aging on DNA methylation and mRNA expression in human liver. EXPERIMENTAL PROCEDURES: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. RESULTS: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151 genes whose liver expression also correlated with age. CONCLUSIONS: We identified age-associated DNA methylation and expression changes in human liver that are partly reflected by epigenetic alterations in blood.
AIM: To study the impact of aging on DNA methylation and mRNA expression in human liver. EXPERIMENTAL PROCEDURES: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. RESULTS: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151 genes whose liver expression also correlated with age. CONCLUSIONS: We identified age-associated DNA methylation and expression changes in human liver that are partly reflected by epigenetic alterations in blood.
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