Literature DB >> 278972

Acetylated histone H4 is preferentially associated with template-active chromatin.

J R Davie, E P Candido.   

Abstract

Chromatin from trout testis at an early stage of development was digested with DNase II (deoxyribonucleate 3'-oligonucleotidohydrolase; EC 3.1.4.6), and the solubilized products were fractionated into Mg2+-soluble and -insoluble components. An examination of the histones from these fractions by one- and two-dimensional polyacrylamide gels showed that the highly acetylated species of histone H4 (di-, tri-, and tetra-acetylated) were associated mainly with the Mg2+-soluble material. Digestion of this chromatin fraction with pancreatic ribonuclease converted more than half of it to an insoluble state, and the acetylated H4 remained associated with the precipitated fraction. No changes in the other histones were noted, but two other basic proteins were also found to be associated with the Mg2+-soluble fraction. Since this fraction is enriched in transcribing gene sequences, it is concluded that the histone H4 of active genes is present in a highly acetylated state.

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Year:  1978        PMID： 278972      PMCID： PMC392827          DOI： 10.1073/pnas.75.8.3574

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.

Authors:  A Garel; R Axel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

3.  Structure of transcriptionally-active chromatin subunits.

Authors:  J M Gottesfeld; P J Butler
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

4.  Chromosomal subunits in active genes have an altered conformation.

Authors:  H Weintraub; M Groudine
Journal:  Science       Date:  1976-09-03       Impact factor: 47.728

5.  Localization of the globin gene in the template active fraction of chromatin of Friend leukemia cells.

Authors:  R B Wallace; S K Dube; J Bonner
Journal:  Science       Date:  1977-12-16       Impact factor: 47.728

6.  Partial purification of the template-active fraction of chromatin: a preliminary report.

Authors:  J M Gottesfeld; W T Garrard; G Bagi; R F Wilson; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

7.  Enzymatic modifications and their possible roles in regulating the binding of basic proteins to DNA and in controlling chromosomal structure.

Authors:  A J Louie; E P Candido; G H Dixon
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

8.  Distribution of messenger RNA-coding sequences in fractionated chromatin.

Authors:  J M Gottesfeld; G A Partington
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

9.  Acetylation and phosphorylation of Drosophila histones. Distribution of acetate and phosphate groups in fractionated chromatin.

Authors:  B Levy-Wilson; R A Gjerset; B J McCarthy
Journal:  Biochim Biophys Acta       Date:  1977-03-02

10.  Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.

Authors:  B Levy W; N C Wong; G H Dixon
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205
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  37 in total

1.  Quantitative PCR analysis of diepoxybutane and epihalohydrin damage to nuclear versus mitochondrial DNA.

Authors:  Frederick J Lariviere; Adam G Newman; Megan L Watts; Sharonda Q Bradley; Justin E Juskewitch; Paul G Greenwood; Julie T Millard
Journal:  Mutat Res       Date:  2009-02-21       Impact factor: 2.433

Review 2.  On the biological role of histone acetylation.

Authors:  A Csordas
Journal:  Biochem J       Date:  1990-01-01       Impact factor: 3.857

3.  Analysis of loss of inactive X chromosomes in interphase cells.

Authors:  J Surrallés; P Jeppesen; H Morrison; A T Natarajan
Journal:  Am J Hum Genet       Date:  1996-11       Impact factor: 11.025

4.  Histone hyperacetylation can induce unfolding of the nucleosome core particle.

Authors:  R Oliva; D P Bazett-Jones; L Locklear; G H Dixon
Journal:  Nucleic Acids Res       Date:  1990-05-11       Impact factor: 16.971

5.  The primary structure of the major isoform (H1.1) of histone H1 from the nematode Caenorhabditis elegans.

Authors:  J R Vanfleteren; S M Van Bun; J J Van Beeumen
Journal:  Biochem J       Date:  1988-10-15       Impact factor: 3.857

6.  Subnuclear fractionation by mild micrococcal-nuclease treatment of nuclei of different transcriptional activities causes a partition of expressed and non-expressed genes.

Authors:  G J Dimitriadis; J R Tata
Journal:  Biochem J       Date:  1980-05-01       Impact factor: 3.857

7.  Post-translational changes of chromosomal proteins in rat cerebellum during postnatal development.

Authors:  I Serra; M Kamiyama; G A Hashim; P Ragonese; B Lombardo; A M Giuffrida
Journal:  Neurochem Res       Date:  1983-12       Impact factor: 3.996

8.  Comparisons of liver chromatin proteins and template activities in parental and heterotic rats during postweaned development.

Authors:  S Amero; J G Tallman; W Kaczmarczyk; V Ulrich
Journal:  Biochem Genet       Date:  1983-06       Impact factor: 1.890

9.  Regulation of nuclear histone acetyltransferase by nucleic acids, histone.DNA complex, and chromatin.

Authors:  L J Wong; D J Sharpe
Journal:  Biochem Genet       Date:  1991-02       Impact factor: 1.890

10.  STAT5 regulation of BCL10 parallels constitutive NFkappaB activation in lymphoid tumor cells.

Authors:  Zsuzsanna S Nagy; Matthew J LeBaron; Jeremy A Ross; Abhisek Mitra; Hallgeir Rui; Robert A Kirken
Journal:  Mol Cancer       Date:  2009-08-26       Impact factor: 27.401
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