| Literature DB >> 27869816 |
Daphne S Bindels1, Lindsay Haarbosch1, Laura van Weeren1, Marten Postma1, Katrin E Wiese1, Marieke Mastop1, Sylvain Aumonier2,3, Guillaume Gotthard2,3, Antoine Royant2,3, Mark A Hink1, Theodorus W J Gadella1.
Abstract
We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.Mesh：
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Year: 2016 PMID： 27869816 DOI： 10.1038/nmeth.4074
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547