| Literature DB >> 27812874 |
Hengrui Zhu1, Linh Le1,2, Hsin-Yao Tang3, David W Speicher3, Rugang Zhang4.
Abstract
Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS), to identify changes in the ubiquitinome in cells that have undergone oncogene-induced senescence.Entities:
Keywords: Liquid chromatography tandem mass spectrometry (LC-MS/MS); Post-translational modification; Senescence; Stable isotope labeling with amino acids in cell culture (SILAC); Ubiquitin; Ubiquitin-proteosome pathway; Ubiquitinome
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Year: 2017 PMID： 27812874 PMCID： PMC5572468 DOI： 10.1007/978-1-4939-6670-7_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745