| Literature DB >> 27812865 |
Michael Shtutman1, Bey-Dih Chang2, Gary P Schools1, Eugenia V Broude3.
Abstract
Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Waf1/Cip1/Sdi1.Entities:
Keywords: HT1080-p21-9; IPTG; Lac-repressor; Senescence; p21Waf1/Cip1/Sdi1
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Year: 2017 PMID： 27812865 PMCID： PMC6764449 DOI： 10.1007/978-1-4939-6670-7_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745