Mohammad El Mouzan1, Feng Wang2, Mohammad Al Mofarreh3, Rajita Menon4, Ahmad Al Barrag5, Kirill S Korolev6, Ahmad Al Sarkhy1, Mona Al Asmi1, Yassin Hamed1, Anjum Saeed1, Scot E Dowd7, Asaad Assiri1, Harland Winter8. 1. Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia. 2. Bioinformatics Program, Boston University, Boston, MA, USA. 3. Al Mofarreh Polyclinic, Department of Gastroenterology, Riyadh, Kingdom of Saudi Arabia. 4. Physics Department, Boston University, Boston, MA, USA. 5. Department of Microbiology, King Saud University, Riyadh, Kingdom of Saudi Arabia. 6. Physics Department and Bioinformatics Program, Boston University, Boston, MA, USA. 7. MRDNA Laboratories, Shallowater, TX, USA. 8. Pediatric IBD Program, MassGeneral Hospital for Children, Boston, MA, USA.
Abstract
Background and Aims: Although increasing evidence suggests a role for fungi in inflammatory bowel disease [IBD], data are scarce and mostly from adults. Our aim was to define the characteristics of fungal microbiota in newly diagnosed treatment-naïve children with Crohn's disease [CD]. Methods: The children referred for colonoscopy were prospectively enrolled in the study at King Khalid University Hospital, King Saud University, and Al Mofarreh Polyclinics in Riyadh. Tissue and stool samples were collected and frozen till sequencing analysis. The children with confirmed CD diagnosis were designated as cases and the others as non- IBD controls; 78 samples were collected from 35 children [15 CD and 20 controls]. Statistical analysis was performed to investigate CD associations and diversity. Results: CD-associated fungi varied with the level of phylogenetic tree. There was no significant difference in abundance between normal and inflamed mucosa. Significantly abundant CD-associated taxa included Psathyrellaceae [p = 0.01], Cortinariaceae [p = 0.04], Psathyrella [p = 0.003], and Gymnopilus [p = 0.03]. Monilinia was significantly depleted [p = 0.03], whereas other depleted taxa, although not statistically significant, included Leotiomycetes [p = 0.06], Helotiales [p = 0.08], and Sclerotiniaceae [p = 0.07]. There was no significant difference in fungal diversity between CD and controls. Conclusions: We report highly significant fungal dysbiosis in newly diagnosed treatment-naïve CD children. Depleted and more abundant taxa suggest anti-inflammatory and pro-inflamatory potentials, respectively. Further studies with larger sample size and including functional analysis are needed to clarify the significance of the fungal community in the pathogenesis of CD.
Background and Aims: Although increasing evidence suggests a role for fungi in inflammatory bowel disease [IBD], data are scarce and mostly from adults. Our aim was to define the characteristics of fungal microbiota in newly diagnosed treatment-naïve children with Crohn's disease [CD]. Methods: The children referred for colonoscopy were prospectively enrolled in the study at King Khalid University Hospital, King Saud University, and Al Mofarreh Polyclinics in Riyadh. Tissue and stool samples were collected and frozen till sequencing analysis. The children with confirmed CD diagnosis were designated as cases and the others as non- IBD controls; 78 samples were collected from 35 children [15 CD and 20 controls]. Statistical analysis was performed to investigate CD associations and diversity. Results: CD-associated fungi varied with the level of phylogenetic tree. There was no significant difference in abundance between normal and inflamed mucosa. Significantly abundant CD-associated taxa included Psathyrellaceae [p = 0.01], Cortinariaceae [p = 0.04], Psathyrella [p = 0.003], and Gymnopilus [p = 0.03]. Monilinia was significantly depleted [p = 0.03], whereas other depleted taxa, although not statistically significant, included Leotiomycetes [p = 0.06], Helotiales [p = 0.08], and Sclerotiniaceae [p = 0.07]. There was no significant difference in fungal diversity between CD and controls. Conclusions: We report highly significant fungal dysbiosis in newly diagnosed treatment-naïve CD children. Depleted and more abundant taxa suggest anti-inflammatory and pro-inflamatory potentials, respectively. Further studies with larger sample size and including functional analysis are needed to clarify the significance of the fungal community in the pathogenesis of CD.
Authors: Mohammad El Mouzan; Abdulrahman Al-Hussaini; Brian Fanelli; Asaad Assiri; Badr AlSaleem; Mohammad Al Mofarreh; Ahmed Al Sarkhy; Mona Alasmi Journal: Dig Dis Sci Date: 2021-03-16 Impact factor: 3.199
Authors: Mohammad I El Mouzan; Kirill S Korolev; Mohammad A Al Mofarreh; Rajita Menon; Harland S Winter; Ahmad A Al Sarkhy; Scot E Dowd; Ahmad M Al Barrag; Asaad A Assiri Journal: World J Gastroenterol Date: 2018-10-21 Impact factor: 5.742
Authors: Kristýna Fiedorová; Matěj Radvanský; Eva Němcová; Hana Grombiříková; Juraj Bosák; Michaela Černochová; Matej Lexa; David Šmajs; Tomáš Freiberger Journal: Front Microbiol Date: 2019-04-17 Impact factor: 5.640