Mohammad El Mouzan1, Abdulrahman Al-Hussaini2, Brian Fanelli3, Asaad Assiri4, Badr AlSaleem5, Mohammad Al Mofarreh6, Ahmed Al Sarkhy7, Mona Alasmi7. 1. Department of Pediatrics (Gastroenterology), King Saud University, P O Box 2925, Riyadh, 11461, Saudi Arabia. melmouzan@ksu.edu.sa. 2. Gastroenterology Division, King Fahad Medical City, Children's Hospital, University of King Saud Bin Abdulaziz for Health Sciences, P. O. Box 59046, Riyadh, 11525, Saudi Arabia. 3. CosmosID, 1600 E Gude Drive, Suite 210, Rockville, MD, 20850, USA. 4. Department of Pediatrics (Gastroenterology) and Supervisor, Prince Abdullah Bin Khalid Celiac Disease Research Chair, King Saud University, P O Box 2925, Riyadh, 11461, Saudi Arabia. 5. Division of Gastroenterology, The Children Hospital, King Fahad Medical City, Pediatric Intestinal Failure and Parenteral Nutrition Program, P. O. Box 59046, Riyadh, 11525, Saudi Arabia. 6. Al Mofarreh PolyClinic, Takhassosi Street, P O Box 9789, Riyadh, 11423, Saudi Arabia. 7. Department of Pediatrics (Gastroenterology), King Saud University, P O Box 2925, Riyadh, 11461, Saudi Arabia.
Abstract
BACKGROUND: Although intestinal fungi are known to interact with the immune system, the relationship between intestinal fungi and childhood celiac disease (CeD), an immune-mediated condition, has rarely been reported. AIMS: The aim of this study was to describe gut fungal profiles in a cohort of children with new-onset CeD. METHODS: Mucosal and fecal samples were collected from children with CeD and controls and subjected to metagenomics analysis of fungal microbiota communities. DNA libraries were sequenced using Illumina HiSeq platform 2 × 150 bp. Bioinformatic analysis was performed to quantify the relative abundance of fungi. Shannon alpha diversity metrics and beta diversity principal coordinate (PCo) analyses were calculated, and DESeq tests were performed between celiac and non-celiac groups. RESULTS: Overall more abundant taxa in samples of children with CeD included Tricholomataceae, Saccharomycetaceae, Saccharomycetes Saccharomyces cerevisiae, and Candida, whereas less abundant taxa included Pichiaceae, Pichia kudriavzevii, Pneumocystis, and Pneumocystis jirovecii. Alpha diversity between CeD and control individuals did not differ significantly, and beta diversity PCo analysis showed overlap of samples from CeD and controls for both fecal or mucosal samples; however, there was a clear separation between mucosal and fecal overall samples CONCLUSIONS: We report fungal dysbiosis in children with CeD, suggesting a possible role in the pathogenesis of CeD. Further larger, controlled, prospective and longitudinal studies are needed to verify the results of this study and clarify the functional role of fungi in CeD.
BACKGROUND: Although intestinal fungi are known to interact with the immune system, the relationship between intestinal fungi and childhood celiac disease (CeD), an immune-mediated condition, has rarely been reported. AIMS: The aim of this study was to describe gut fungal profiles in a cohort of children with new-onset CeD. METHODS: Mucosal and fecal samples were collected from children with CeD and controls and subjected to metagenomics analysis of fungal microbiota communities. DNA libraries were sequenced using Illumina HiSeq platform 2 × 150 bp. Bioinformatic analysis was performed to quantify the relative abundance of fungi. Shannon alpha diversity metrics and beta diversity principal coordinate (PCo) analyses were calculated, and DESeq tests were performed between celiac and non-celiac groups. RESULTS: Overall more abundant taxa in samples of children with CeD included Tricholomataceae, Saccharomycetaceae, Saccharomycetes Saccharomyces cerevisiae, and Candida, whereas less abundant taxa included Pichiaceae, Pichia kudriavzevii, Pneumocystis, and Pneumocystis jirovecii. Alpha diversity between CeD and control individuals did not differ significantly, and beta diversity PCo analysis showed overlap of samples from CeD and controls for both fecal or mucosal samples; however, there was a clear separation between mucosal and fecal overall samples CONCLUSIONS: We report fungal dysbiosis in children with CeD, suggesting a possible role in the pathogenesis of CeD. Further larger, controlled, prospective and longitudinal studies are needed to verify the results of this study and clarify the functional role of fungi in CeD.
Authors: Mohammad El Mouzan; Feng Wang; Mohammad Al Mofarreh; Rajita Menon; Ahmad Al Barrag; Kirill S Korolev; Ahmad Al Sarkhy; Mona Al Asmi; Yassin Hamed; Anjum Saeed; Scot E Dowd; Asaad Assiri; Harland Winter Journal: J Crohns Colitis Date: 2017-05-01 Impact factor: 10.020
Authors: Mohammad El Mouzan; Asaad Assiri; Ahmed Al Sarkhy; Mona Alasmi; Anjum Saeed; Abdulrahman Al-Hussaini; Badr AlSaleem; Mohammad Al Mofarreh Journal: PLoS One Date: 2022-01-14 Impact factor: 3.240
Authors: Rebecka Ventin-Holmberg; Schahzad Saqib; Katri Korpela; Anne Nikkonen; Ville Peltola; Anne Salonen; Willem M de Vos; Kaija-Leena Kolho Journal: J Fungi (Basel) Date: 2022-03-22