| Literature DB >> 27798610 |
Bryce T Bajar1,2,3, Amy J Lam1,2, Ryan K Badiee4, Young-Hee Oh1,2,5, Jun Chu1,2, Xin X Zhou1,2,5, Namdoo Kim1,2,5, Benjamin B Kim1,2,5, Mingyu Chung6, Arielle L Yablonovitch7, Barney F Cruz8, Kanokwan Kulalert9, Jacqueline J Tao1, Tobias Meyer6, Xiao-Dong Su3, Michael Z Lin1,2,5.
Abstract
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.Entities:
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Year: 2016 PMID： 27798610 PMCID： PMC5548384 DOI： 10.1038/nmeth.4045
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547