Enrico Ammirati1, Francesco Moroni2, Marco Magnoni3, Simona Di Terlizzi4, Chiara Villa4, Federico Sizzano5, Alessio Palini5, Katia Garlaschelli6, Fernanda Tripiciano7, Isabella Scotti8, Alberico Luigi Catapano9, Angelo A Manfredi10, Giuseppe Danilo Norata11, Paolo G Camici3. 1. Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy; De Gasperis Cardio Center, Niguarda Ca' Granda Hospital, Milan, Italy. Electronic address: ammirati.enrico@hsr.it. 2. Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy. Electronic address: moroni@studenti.unisr.it. 3. Cardiothoracic Department, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy. 4. FRACTAL - Flow cytometry Resource Advanced Cytometry Technical Applications Laboratory, San Raffaele Scientific Institute, Milan, Italy. 5. Nestlé Institute of Health Sciences, Biobanking & Flow Cytometry Core EPFL, Innovation Park Bâtiment H, Lausanne, Switzerland. 6. Center SISA for the Study of Atherosclerosis, Bassini Hospital, Cinisello Balsamo, Italy. 7. Hematology and Blood Transfusion Service, San Raffaele Scientific Institute, Milan, Italy. 8. Department of Rheumatology, Istituto Ortopedico Gaetano Pini, Milan, Italy. 9. IRCCS - Multimedica Hospital, Sesto San Giovanni, Italy; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy. 10. Unit of Internal Medicine & Clinical Immunology, San Raffaele Scientific Institute and Vita-Salute University, Milan, Italy. 11. Center SISA for the Study of Atherosclerosis, Bassini Hospital, Cinisello Balsamo, Italy; Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy; School of Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia.
Abstract
BACKGROUND AND AIMS: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. METHODS: Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. RESULTS: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). CONCLUSIONS: Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored. Copyright Â
BACKGROUND AND AIMS: Monocytes are known to play a key role in the initiation and progression of atherosclerosis and contribute to plaque destabilization through the generation of signals that promote inflammation and neoangiogenesis. In humans, studies investigating the features of circulating monocytes in advanced atherosclerotic lesions are lacking. METHODS:Patients (mean age 69 years, 56% males) with intermediate asymptomatic carotid stenosis (40-70% in diameter) were evaluated for maximal stenosis in common carotid artery, carotid bulb and internal carotid artery, overall disease burden as estimated with total plaque area (TPA), greyscale and neovascularization in 244 advanced carotid plaques. Absolute counts of circulating CD14+ monocytes, of classical (CD14highCD16-), intermediate (CD14highCD16+) and non-classical (CD14lowCD16+) monocytes and HLA-DR+ median fluorescence intensity for each subset were evaluated with flow cytometry. RESULTS: No correlation was found between monocytes and overall atherosclerotic burden, nor with high sensitivity C-reactive protein (hsCRP) or interleukin-6 (IL-6). In contrast, plaque signs of neovascularization were associated with significantly lower counts of circulating CD14+ monocytes (297 versus 350 cells/mm3, p = 0.039) and of classical monocytes (255 versus 310 cells/mm3, p = 0.029). CONCLUSIONS:Neovascularized atherosclerotic lesions selectively associate with lower blood levels of CD14+ and CD14highCD16- monocytes independently of systemic inflammatory activity, as indicated by normal hsCRP levels. Whether the reduction of circulating CD14+ and CD14highCD16- monocytes is due to a potential redistribution of these cell types into active lesions remains to be explored. Copyright Â
Authors: Enrico Ammirati; Francesco Moroni; Marco Magnoni; Maria A Rocca; Roberta Messina; Nicoletta Anzalone; Costantino De Filippis; Isabella Scotti; Francesca Besana; Pietro Spagnolo; Ornella E Rimoldi; Roberto Chiesa; Andrea Falini; Massimo Filippi; Paolo G Camici Journal: Int J Cardiol Heart Vasc Date: 2020-08-20
Authors: L Saba; S Zucca; A Gupta; G Micheletti; J S Suri; A Balestrieri; M Porcu; P Crivelli; G Lanzino; Y Qi; V Nardi; G Faa; R Montisci Journal: AJNR Am J Neuroradiol Date: 2020-07-30 Impact factor: 3.825
Authors: Enrico Ammirati; Francesco Moroni; Marco Magnoni; Maria A Rocca; Roberta Messina; Nicoletta Anzalone; Costantino De Filippis; Isabella Scotti; Francesca Besana; Pietro Spagnolo; Ornella E Rimoldi; Roberto Chiesa; Andrea Falini; Massimo Filippi; Paolo G Camici Journal: Sci Rep Date: 2017-09-05 Impact factor: 4.379
Authors: Enrico Ammirati; Francesco Moroni; Marco Magnoni; Elena Busnardo; Simona Di Terlizzi; Chiara Villa; Federico Sizzano; Isabella Scotti; Alessio Palini; Luca Presotto; Valentino Bettinardi; Pietro Spagnolo; Francesca Besana; Luigi Gianolli; Ornella E Rimoldi; Paolo G Camici Journal: Int J Cardiol Heart Vasc Date: 2018-09-25
Authors: Theodore S Kapellos; Lorenzo Bonaguro; Ioanna Gemünd; Nico Reusch; Adem Saglam; Emily R Hinkley; Joachim L Schultze Journal: Front Immunol Date: 2019-08-30 Impact factor: 7.561