Francesco De Francesco1, Antonio Graziano2,3, Letizia Trovato2, Gabriele Ceccarelli4,5, Maurizio Romano6, Marco Marcarelli7, Gabriella Maria Cusella De Angelis4,5, Umberto Cillo6, Michele Riccio8, Giuseppe Andrea Ferraro9. 1. Multidisciplinary Department of Medical-Surgical and Dental Specialties, Second University of Naples, Miraglia Square, 80122, Naples, Italy. fran.defr@libero.it. 2. Human Brain Wave srl, Corso Galileo Ferraris 63, 10128, Torino, Italy. 3. SHRO - Temple University of Philadelphia, Philadelphia, PA, USA. 4. Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia, Italy. 5. C.I.T., Tissue Engineering Centre, University of Pavia, Pavia, Italy. 6. Department of Surgery, Oncology and Gastroenterology, Hepatobiliary Surgery and Liver Transplantation, Padua University Hospital, Padua, Italy. 7. Moncalieri Hospital, ASL To5 - U.C.S. Orthopedics and Traumatology, Piazza A. Ferdinando, 3, 10024, Moncalieri TO, Italy. 8. Department of Reconstructive Plastic Surgery-Hand Surgery, AOU "Ospedali Riuniti", Ancona, Italy. 9. Multidisciplinary Department of Medical-Surgical and Dental Specialties, Second University of Naples, Miraglia Square, 80122, Naples, Italy.
Abstract
BACKGROUND: The etiology of non-healing ulcers depends on both systemic and local factors. The introduction of advanced dressing, negative wound therapy and compression therapy have undoubtedly improved clinical outcomes. The principal aim of study was to demonstrate the efficacy of dermal micrografts in the treatment of ulcers with different etiologies. The second aim was to investigate in vitro the action of micrografts in the regenerative process. METHODS: The dermal micro-grafts were obtained from mechanical disaggregation of small pieces of skin tissue through a medical device called Rigeneracons. RESULTS: We observed in vivo the ability of dermal autologous micrografts to improve the healing of venous, diabetic, pressure and post-traumatic ulcers after few week of treatment accomplished in general with a better quality of life for the patients. In vitro results showed that these micrografts express mesenchymal stem cells (MSCS) marker such as CD34, CD73, CD90 and CD105, and are able to form a viable and proliferative biocomplex with collagen sponge. Finally, the site of ulcers displayed a different expression of epidermal growth factors, insulin-like growth factors, platelet-derived growth factors and their receptors and tumor necrosis factor-β with respect to healthy skin samples. CONCLUSION: We reported a good outcome for the treatment of chronic ulcers using dermal autologous micrografts. Finally, we suggest that the positivity to MSCs markers and the ability to interact with a scaffold can play a key role in their regenerative properties.
BACKGROUND: The etiology of non-healing ulcers depends on both systemic and local factors. The introduction of advanced dressing, negative wound therapy and compression therapy have undoubtedly improved clinical outcomes. The principal aim of study was to demonstrate the efficacy of dermal micrografts in the treatment of ulcers with different etiologies. The second aim was to investigate in vitro the action of micrografts in the regenerative process. METHODS: The dermal micro-grafts were obtained from mechanical disaggregation of small pieces of skin tissue through a medical device called Rigeneracons. RESULTS: We observed in vivo the ability of dermal autologous micrografts to improve the healing of venous, diabetic, pressure and post-traumatic ulcers after few week of treatment accomplished in general with a better quality of life for the patients. In vitro results showed that these micrografts express mesenchymal stem cells (MSCS) marker such as CD34, CD73, CD90 and CD105, and are able to form a viable and proliferative biocomplex with collagen sponge. Finally, the site of ulcers displayed a different expression of epidermal growth factors, insulin-like growth factors, platelet-derived growth factors and their receptors and tumor necrosis factor-β with respect to healthy skin samples. CONCLUSION: We reported a good outcome for the treatment of chronic ulcers using dermal autologous micrografts. Finally, we suggest that the positivity to MSCs markers and the ability to interact with a scaffold can play a key role in their regenerative properties.
Authors: Fabiano Svolacchia; Francesco De Francesco; Letizia Trovato; Antonio Graziano; Giuseppe A Ferraro Journal: J Cosmet Dermatol Date: 2016-01-30 Impact factor: 2.696
Authors: Natalia Vapniarsky; Boaz Arzi; Jerry C Hu; Jan A Nolta; Kyriacos A Athanasiou Journal: Stem Cells Transl Med Date: 2015-08-07 Impact factor: 6.940
Authors: Gabriele Ceccarelli; Pietro Gentile; Marco Marcarelli; Martina Balli; Flavio Lorenzo Ronzoni; Laura Benedetti; Maria Gabriella Cusella De Angelis Journal: Pharmaceuticals (Basel) Date: 2017-06-13
Authors: Elena Dai Prè; Alice Busato; Silvia Mannucci; Federica Vurro; Francesco De Francesco; Valentina Riccio; Samantha Solito; Reetuparna Biswas; Paolo Bernardi; Michele Riccio; Andrea Sbarbati Journal: Int J Mol Sci Date: 2020-04-27 Impact factor: 5.923
Authors: Marco Marcarelli; Marcello Zappia; Lorenzo Rissolio; Chiara Baroni; Carlo Astarita; Letizia Trovato; Antonio Graziano Journal: J Clin Med Date: 2021-01-17 Impact factor: 4.241
Authors: Marco Mario Tresoldi; Antonio Graziano; Alberto Malovini; Angela Faga; Giovanni Nicoletti Journal: Stem Cells Int Date: 2019-09-08 Impact factor: 5.443
Authors: Michele Riccio; Andrea Marchesini; Nicola Zingaretti; Sara Carella; Letizia Senesi; Maria Giuseppina Onesti; Pier Camillo Parodi; Diego Ribuffo; Luca Vaienti; Francesco De Francesco Journal: Stem Cells Int Date: 2019-12-01 Impact factor: 5.443