Literature DB >> 27709565

Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.

Ciaran M Lee1, Haibao Zhu1, Timothy H Davis1, Harshahardhan Deshmukh1, Gang Bao2.   

Abstract

The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.

Entities:  

Keywords:  CRISPR; Genome editing; Targeted gene knockout

Mesh：

Year:  2017        PMID： 27709565     DOI： 10.1007/978-1-4939-6472-7_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies.

Authors:  Diogo Gomes-Silva; Madhuwanti Srinivasan; Sandhya Sharma; Ciaran M Lee; Dimitrios L Wagner; Timothy H Davis; Rayne H Rouce; Gang Bao; Malcolm K Brenner; Maksim Mamonkin
Journal:  Blood       Date:  2017-05-24       Impact factor: 22.113

Review 2.  Examination of CRISPR/Cas9 design tools and the effect of target site accessibility on Cas9 activity.

Authors:  Ciaran M Lee; Timothy H Davis; Gang Bao
Journal:  Exp Physiol       Date:  2017-04-12       Impact factor: 2.969

3.  Understanding the Mechanism of Dysglycemia in a Fanconi-Bickel Syndrome Patient.

Authors:  Sanaa Sharari; Mustapha Aouida; Idris Mohammed; Basma Haris; Ajaz Ahmad Bhat; Iman Hawari; Sabah Nisar; Igor Pavlovski; Kabir H Biswas; Najeeb Syed; Selma Maacha; Jean-Charles Grivel; Maryam Saifaldeen; Johan Ericsson; Khalid Hussain
Journal:  Front Endocrinol (Lausanne)       Date:  2022-05-18       Impact factor: 6.055

4.  A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system.

Authors:  Mustapha Aouida; Dina Aljogol; Reem Ali; Dindial Ramotar
Journal:  STAR Protoc       Date:  2022-03-04

5.  Two efficient CRISPR/Cas9 systems for gene editing in soybean.

Authors:  Jéssica Carrijo; Eudald Illa-Berenguer; Peter LaFayette; Nathalia Torres; Francisco J L Aragão; Wayne Parrott; Giovanni R Vianna
Journal:  Transgenic Res       Date:  2021-04-02       Impact factor: 2.788
  5 in total

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