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Literature DB >> 27699132

3D imaging in CUBIC-cleared mouse heart tissue: going deeper.

Imke Nehrhoff¹, Diana Bocancea², Javier Vaquero³, Juan José Vaquero⁴, Jorge Ripoll⁴, Manuel Desco⁵, María Victoria Gómez-Gaviro⁴.

Abstract

The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.

Entities: Disease Species

Keywords: (000.1430) Biology and medicine; (180.1790) Confocal microscopy

Year: 2016 PMID： 27699132 PMCID： PMC5030044 DOI： 10.1364/BOE.7.003716

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

8 in total

1. Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging.

Authors: Etsuo A Susaki; Kazuki Tainaka; Dimitri Perrin; Hiroko Yukinaga; Akihiro Kuno; Hiroki R Ueda
Journal: Nat Protoc Date: 2015-10-08 Impact factor: 13.491

2. Cardiac muscle organization revealed in 3-D by imaging whole-mount mouse hearts using two-photon fluorescence and confocal microscopy.

Authors: Mayandi Sivaguru; Glenn Fried; Barghav S Sivaguru; Vignesh A Sivaguru; Xiaochen Lu; Kyung Hwa Choi; M Taher A Saif; Brian Lin; Sakthivel Sadayappan
Journal: Biotechniques Date: 2015-11-01 Impact factor: 1.993

3. Mechanotransduction Mechanisms for Intraventricular Diastolic Vortex Forces and Myocardial Deformations: Part 2.

Authors: Ares Pasipoularides
Journal: J Cardiovasc Transl Res Date: 2015-05-14 Impact factor: 4.132

4. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.

Authors: Etsuo A Susaki; Kazuki Tainaka; Dimitri Perrin; Fumiaki Kishino; Takehiro Tawara; Tomonobu M Watanabe; Chihiro Yokoyama; Hirotaka Onoe; Megumi Eguchi; Shun Yamaguchi; Takaya Abe; Hiroshi Kiyonari; Yoshihiro Shimizu; Atsushi Miyawaki; Hideo Yokota; Hiroki R Ueda
Journal: Cell Date: 2014-04-17 Impact factor: 41.582

Review 5. Mechanotransduction mechanisms for intraventricular diastolic vortex forces and myocardial deformations: part 1.

Authors: Ares Pasipoularides
Journal: J Cardiovasc Transl Res Date: 2015-01-27 Impact factor: 4.132

Review 6. Clarifying Tissue Clearing.

Authors: Douglas S Richardson; Jeff W Lichtman
Journal: Cell Date: 2015-07-16 Impact factor: 41.582

7. High-resolution visualization of mouse cardiac microvasculature using optical histology.

Authors: Austin J Moy; Patrick C Lo; Bernard Choi
Journal: Biomed Opt Express Date: 2013-12-04 Impact factor: 3.732

8. A global double-fluorescent Cre reporter mouse.

Authors: Mandar Deepak Muzumdar; Bosiljka Tasic; Kazunari Miyamichi; Ling Li; Liqun Luo
Journal: Genesis Date: 2007-09 Impact factor: 2.487

8 in total

10 in total

1. Imaging the brain in 3D using a combination of CUBIC and immunofluorescence staining.

Authors: Yangyang Xu; Peng Li; Mengqi Wang; Jie Zhang; Wei Wang
Journal: Biomed Opt Express Date: 2019-04-01 Impact factor: 3.732

2. Looking inside the heart: a see-through view of the vascular tree.

Authors: Imke Nehrhoff; Jorge Ripoll; Rafael Samaniego; Manuel Desco; Maria Victoria Gómez-Gaviro
Journal: Biomed Opt Express Date: 2017-05-30 Impact factor: 3.732

3. Imaging transparent intact cardiac tissue with single-cell resolution.

Authors: Zhiwei Wang; Jie Zhang; Guangpu Fan; Hui Zhao; Xu Wang; Jing Zhang; Peide Zhang; Wei Wang
Journal: Biomed Opt Express Date: 2018-01-04 Impact factor: 3.732

4. Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts.

Authors: Demetria M Fischesser; Evan C Meyer; Michelle Sargent; Jeffery D Molkentin
Journal: J Vis Exp Date: 2021-05-16 Impact factor: 1.355

5. CUBIC pathology: three-dimensional imaging for pathological diagnosis.

Authors: Satoshi Nojima; Etsuo A Susaki; Kyotaro Yoshida; Hiroyoshi Takemoto; Naoto Tsujimura; Shohei Iijima; Ko Takachi; Yujiro Nakahara; Shinichiro Tahara; Kenji Ohshima; Masako Kurashige; Yumiko Hori; Naoki Wada; Jun-Ichiro Ikeda; Atsushi Kumanogoh; Eiichi Morii; Hiroki R Ueda
Journal: Sci Rep Date: 2017-08-24 Impact factor: 4.379

6. Quantification of sympathetic hyperinnervation and denervation after myocardial infarction by three-dimensional assessment of the cardiac sympathetic network in cleared transparent murine hearts.

Authors: Teruki Yokoyama; Jong-Kook Lee; Keiko Miwa; Tobias Opthof; Satoki Tomoyama; Hiroyuki Nakanishi; Akira Yoshida; Haruyo Yasui; Tadatsune Iida; Shigeru Miyagawa; Shigeo Okabe; Yoshiki Sawa; Yasushi Sakata; Issei Komuro
Journal: PLoS One Date: 2017-07-28 Impact factor: 3.240

7. Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies.

Authors: Pradeep S Rajendran; Rosemary C Challis; Charless C Fowlkes; Peter Hanna; John D Tompkins; Maria C Jordan; Sarah Hiyari; Beth A Gabris-Weber; Alon Greenbaum; Ken Y Chan; Benjamin E Deverman; Heike Münzberg; Jeffrey L Ardell; Guy Salama; Viviana Gradinaru; Kalyanam Shivkumar
Journal: Nat Commun Date: 2019-04-26 Impact factor: 14.919