| Literature DB >> 24746791 |
Etsuo A Susaki1, Kazuki Tainaka2, Dimitri Perrin3, Fumiaki Kishino4, Takehiro Tawara5, Tomonobu M Watanabe6, Chihiro Yokoyama7, Hirotaka Onoe7, Megumi Eguchi8, Shun Yamaguchi9, Takaya Abe10, Hiroshi Kiyonari10, Yoshihiro Shimizu11, Atsushi Miyawaki12, Hideo Yokota5, Hiroki R Ueda13.
Abstract
Systems-level identification and analysis of cellular circuits in the brain will require the development of whole-brain imaging with single-cell resolution. To this end, we performed comprehensive chemical screening to develop a whole-brain clearing and imaging method, termed CUBIC (clear, unobstructed brain imaging cocktails and computational analysis). CUBIC is a simple and efficient method involving the immersion of brain samples in chemical mixtures containing aminoalcohols, which enables rapid whole-brain imaging with single-photon excitation microscopy. CUBIC is applicable to multicolor imaging of fluorescent proteins or immunostained samples in adult brains and is scalable from a primate brain to subcellular structures. We also developed a whole-brain cell-nuclear counterstaining protocol and a computational image analysis pipeline that, together with CUBIC reagents, enable the visualization and quantification of neural activities induced by environmental stimulation. CUBIC enables time-course expression profiling of whole adult brains with single-cell resolution.Entities:
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Year: 2014 PMID： 24746791 DOI： 10.1016/j.cell.2014.03.042
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582