Literature DB >> 27688368

A Fast Method for DEFB1 - 44C/G SNP Genotyping in Brazilian Patients with Periodontitis.

Renata Cimőes1, Rafael Rafael Amorim Cavalcanti de Siqueira2, Sergio Crovella1, Paulo Roberto Eleutério de Souza3, Nikos Donos4.   

Abstract

AIM: Defensins are cationic antimicrobial peptides expressed in epithelial cells. Such peptides exhibit antibacterial, antifungal and antiviral properties, and are a component of the innate immune response. It has been suggested that they have a protective role in the oral cavity. This study evaluated the DEFB1 polymorphism in diabetic patients with or without periodontitis in comparison to healthy controls.
MATERIAL AND METHODS: We used Hairpin-Shaped Primer (HP) assay to study the distribution of the -44 C/G SNP (rs1800972) in 119 human DNAs obtained from diabetic patients and healthy control patients.
RESULTS: The results indicate that there are no differences in distribution between groups and that in diabetic periodontitis patients the homozygous mutant could be found more frequently.
CONCLUSION: Further studies are necessary in order to investigate the role of DEFB1 polymorphisms in diabetic periodontitis patients and the influence of the peptide in periodontal pathogens.

Entities:  

Keywords:  Antimicrobial Cationic Peptides; DEFB1 Protein; Defensins; Diabetes Complications; Epithelial Cells; Humans; Periodontitis

Year:  2014        PMID: 27688368      PMCID: PMC4872829          DOI: 10.15644/asc48/3/5

Source DB:  PubMed          Journal:  Acta Stomatol Croat        ISSN: 0001-7019


  28 in total

Review 1.  Antimicrobial peptides and proteins in the innate defense of the airway surface.

Authors:  S M Travis; P K Singh; M J Welsh
Journal:  Curr Opin Immunol       Date:  2001-02       Impact factor: 7.486

Review 2.  Single nucleotide polymorphisms as tools in human genetics.

Authors:  I C Gray; D A Campbell; N K Spurr
Journal:  Hum Mol Genet       Date:  2000-10       Impact factor: 6.150

3.  DEFB1 gene polymorphisms and increased risk of HIV-1 infection in Brazilian children.

Authors:  Michele Milanese; Ludovica Segat; Alessandra Pontillo; Luiz Claudio Arraes; José Luiz de Lima Filho; Sergio Crovella
Journal:  AIDS       Date:  2006-08-01       Impact factor: 4.177

4.  Transcriptional effect of DEFB1 gene 5' untranslated region polymorphisms.

Authors:  Michele Milanese; Ludovica Segat; Sergio Crovella
Journal:  Cancer Res       Date:  2007-06-15       Impact factor: 12.701

5.  Prevalence and risk indicators for periodontal attachment loss in a population of older community-dwelling blacks and whites.

Authors:  J D Beck; G G Koch; R G Rozier; G E Tudor
Journal:  J Periodontol       Date:  1990-08       Impact factor: 6.993

6.  Discovery of new human beta-defensins using a genomics-based approach.

Authors:  H P Jia; B C Schutte; A Schudy; R Linzmeier; J M Guthmiller; G K Johnson; B F Tack; J P Mitros; A Rosenthal; T Ganz; P B McCray
Journal:  Gene       Date:  2001-01-24       Impact factor: 3.688

7.  Human beta-defensin-1, a potential chromosome 8p tumor suppressor: control of transcription and induction of apoptosis in renal cell carcinoma.

Authors:  Carrie Q Sun; Rebecca Arnold; Carina Fernandez-Golarz; Amanda B Parrish; Tara Almekinder; Ju He; Shuk-mei Ho; Pavel Svoboda; Jan Pohl; Fray F Marshall; John A Petros
Journal:  Cancer Res       Date:  2006-09-01       Impact factor: 12.701

8.  A single-nucleotide polymorphism in the human beta-defensin 1 gene is associated with HIV-1 infection in Italian children.

Authors:  Laura Braida; Michele Boniotto; Alessandra Pontillo; Pier Angelo Tovo; Antonio Amoroso; Sergio Crovella
Journal:  AIDS       Date:  2004-07-23       Impact factor: 4.177

9.  Single nucleotide polymorphisms and the haplotype in the DEFB1 gene are associated with atopic dermatitis in a Korean population.

Authors:  Eugene Kim; Jong-Eun Lee; Jung-Hyun Namkung; Pyoung-Su Kim; Sook Kim; Eun-Soon Shin; Eun-Young Cho; Jun-Mo Yang
Journal:  J Dermatol Sci       Date:  2009-01-09       Impact factor: 4.563

10.  Polymorphisms of the human beta-defensin-1 gene.

Authors:  T Dörk; M Stuhrmann
Journal:  Mol Cell Probes       Date:  1998-06       Impact factor: 2.365

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