Cristina O'Callaghan-Gordo1,2,3, Manolis Kogevinas4,5,6,7, Marie Pedersen4,5,6,8,9,10, Eleni Fthenou11, Ana Espinosa4,5,6,7, Xristina Tsiapa4,5,6,11, Georgia Chalkiadaki11, Vasiliki Daraki11, Eirini Dermitzaki11, Ilse Decordier12, Peter B Farmer13, Panagiotis Georgiadis14, Vaggelis Georgiou11, Soterios A Kyrtopoulos14, Domenico Franco Merlo15, Dora Romaguera4,5,6,16, Theano Roumeliotaki11, Katerina Sarri11, Margareta Törnqvist17, Kim Vande Loock12, Hans von Stedingk17, Jos Kleinjans18, Micheline Kirsch-Volders12, Leda Chatzi11. 1. ISGlobal, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain. cristina.ocallaghan@isglobal.org. 2. Universitat Pompeu Fabra (UPF), Barcelona, Spain. cristina.ocallaghan@isglobal.org. 3. CIBER Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. cristina.ocallaghan@isglobal.org. 4. ISGlobal, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain. 5. Universitat Pompeu Fabra (UPF), Barcelona, Spain. 6. CIBER Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. 7. IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain. 8. National Institute of Health and Medical Research (INSERM), U823, Team of Environmental Epidemiology Applied to Reproduction and Respiratory Health, Institute Albert Bonniot, Grenoble, France. 9. Department of Public Health, Centre for Epidemiology and Screening CSS, University of Copenhagen, Copenhagen, Denmark. 10. Diet, Genes and Environment, Danish Cancer Society Research Center, Copenhagen, Denmark. 11. Faculty of Medicine, University of Crete, Heraklion, Greece. 12. Laboratory of Cell Genetics, Faculty of Science and Bio-engineering, Vrije Universitei Brussel, Brussels, Belgium. 13. Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK. 14. National Hellenic Research Foundation, Institute of Biology, Medicinal Chemistry and Biotechnology, Athens, Greece. 15. Epidemiology, Biostatistics, and Clinical Trials, Istituto di Ricerca e Cura a Carattere Scientifico (IRCCS) Azienda Ospedaliera Universitaria (AOU) San Martino-Istituto Nazionale per la Ricerca sul Cancro (IST), Genoa, Italy. 16. CIBER Fisiopatología de la Obesidad y Nutrición, CIBER-OBN, Madrid, Spain. 17. Department of Environmental Science and Analytical Chemistry, Stockholm University, Stockholm, Sweden. 18. Department of Toxicogenomics, Maastricht University, Maastricht, The Netherlands.
Abstract
PURPOSE: The study assessed whether diet and adherence to cancer prevention guidelines during pregnancy were associated with micronucleus (MN) frequency in mothers and newborns. MN is biomarkers of early genetic effects that have been associated with cancer risk in adults. METHODS: A total of 188 mothers and 200 newborns from the Rhea cohort (Greece) were included in the study. At early-mid pregnancy, we conducted personal interviews and a validated food frequency questionnaire was completed. With this information, we constructed a score reflecting adherence to the World Cancer Research Fund/American Institute for Cancer Research cancer prevention guidelines on diet, physical activity and body fatness. At delivery, maternal and/or cord blood was collected to measure DNA and hemoglobin adducts of dietary origin and frequencies of MN in binucleated and mononucleated T lymphocytes (MNBN and MNMONO). RESULTS: In mothers, higher levels of red meat consumption were associated with increased MNBN frequency [2nd tertile IRR = 1.34 (1.00, 1.80), 3rd tertile IRR = 1.33 (0.96, 1.85)] and MNMONO frequency [2nd tertile IRR = 1.53 (0.84, 2.77), 3rd tertile IRR = 2.69 (1.44, 5.05)]. The opposite trend was observed for MNBN in newborns [2nd tertile IRR = 0.64 (0.44, 0.94), 3rd tertile IRR = 0.68 (0.46, 1.01)], and no association was observed with MNMONO. Increased MN frequency in pregnant women with high red meat consumption is consistent with previous knowledge. CONCLUSIONS: Our results also suggest exposure to genotoxics during pregnancy might affect differently mothers and newborns. The predictive value of MN as biomarker for childhood cancer, rather than adulthood, remains unclear. With few exceptions, the association between maternal carcinogenic exposures during pregnancy and childhood cancer or early biologic effect biomarkers remains poorly understood.
PURPOSE: The study assessed whether diet and adherence to cancer prevention guidelines during pregnancy were associated with micronucleus (MN) frequency in mothers and newborns. MN is biomarkers of early genetic effects that have been associated with cancer risk in adults. METHODS: A total of 188 mothers and 200 newborns from the Rhea cohort (Greece) were included in the study. At early-mid pregnancy, we conducted personal interviews and a validated food frequency questionnaire was completed. With this information, we constructed a score reflecting adherence to the World Cancer Research Fund/American Institute for Cancer Research cancer prevention guidelines on diet, physical activity and body fatness. At delivery, maternal and/or cord blood was collected to measure DNA and hemoglobin adducts of dietary origin and frequencies of MN in binucleated and mononucleated T lymphocytes (MNBN and MNMONO). RESULTS: In mothers, higher levels of red meat consumption were associated with increased MNBN frequency [2nd tertile IRR = 1.34 (1.00, 1.80), 3rd tertile IRR = 1.33 (0.96, 1.85)] and MNMONO frequency [2nd tertile IRR = 1.53 (0.84, 2.77), 3rd tertile IRR = 2.69 (1.44, 5.05)]. The opposite trend was observed for MNBN in newborns [2nd tertile IRR = 0.64 (0.44, 0.94), 3rd tertile IRR = 0.68 (0.46, 1.01)], and no association was observed with MNMONO. Increased MN frequency in pregnant women with high red meat consumption is consistent with previous knowledge. CONCLUSIONS: Our results also suggest exposure to genotoxics during pregnancy might affect differently mothers and newborns. The predictive value of MN as biomarker for childhood cancer, rather than adulthood, remains unclear. With few exceptions, the association between maternal carcinogenic exposures during pregnancy and childhood cancer or early biologic effect biomarkers remains poorly understood.
Authors: Dora Romaguera; Anne-Claire Vergnaud; Petra H Peeters; Carla H van Gils; Doris S M Chan; Pietro Ferrari; Isabelle Romieu; Mazda Jenab; Nadia Slimani; Françoise Clavel-Chapelon; Guy Fagherazzi; Florence Perquier; Rudolf Kaaks; Birgit Teucher; Heiner Boeing; Anne von Rüsten; Anne Tjønneland; Anja Olsen; Christina C Dahm; Kim Overvad; José Ramón Quirós; Carlos A Gonzalez; María José Sánchez; Carmen Navarro; Aurelio Barricarte; Miren Dorronsoro; Kay-Tee Khaw; Nicholas J Wareham; Francesca L Crowe; Timothy J Key; Antonia Trichopoulou; Pagona Lagiou; Christina Bamia; Giovanna Masala; Paolo Vineis; Rosario Tumino; Sabina Sieri; Salvatore Panico; Anne M May; H Bas Bueno-de-Mesquita; Frederike L Büchner; Elisabet Wirfält; Jonas Manjer; Ingegerd Johansson; Göran Hallmans; Guri Skeie; Kristin Benjaminsen Borch; Christine L Parr; Elio Riboli; Teresa Norat Journal: Am J Clin Nutr Date: 2012-05-16 Impact factor: 7.045
Authors: Chelsea E Catsburg; Manuela Gago-Dominguez; Jian-Min Yuan; J Esteban Castelao; Victoria K Cortessis; Malcolm C Pike; Mariana C Stern Journal: Int J Cancer Date: 2013-07-15 Impact factor: 7.396
Authors: Domenico Franco Merlo; Silvia Agramunt; Lívia Anna; Harrie Besselink; Maria Botsivali; Nigel J Brady; Marcello Ceppi; Leda Chatzi; Bowang Chen; Ilse Decordier; Peter B Farmer; Sarah Fleming; Vincenzo Fontana; Asta Försti; Eleni Fthenou; Fabio Gallo; Panagiotis Georgiadis; Hans Gmuender; Roger W Godschalk; Berit Granum; Laura J Hardie; Kari Hemminki; Kevin Hochstenbach; Lisbeth E Knudsen; Manolis Kogevinas; Katalin Kovács; Soterios A Kyrtopoulos; Martinus Løvik; Jeanette K Nielsen; Unni Cecilie Nygaard; Marie Pedersen; Per Rydberg; Bernadette Schoket; Dan Segerbäck; Rajinder Singh; Jordi Sunyer; Margareta Törnqvist; Henk van Loveren; Frederik J van Schooten; Kim Vande Loock; Hans von Stedingk; John Wright; Jos C Kleinjans; Micheline Kirsch-Volders; Joost H M van Delft Journal: Environ Health Perspect Date: 2013-11-19 Impact factor: 9.031
Authors: Aleksandra Fucic; Mirta Starcevic; Nada Sindicic Dessardo; Drago Batinic; Sasa Kralik; Jure Krasic; Nino Sincic; Damir Loncarevic; Vedrana Guszak Journal: Int J Environ Res Public Health Date: 2020-05-13 Impact factor: 3.390